Colony hybridizations using nylon membranes and RNA probes - an improved method for screening bacterial clones containing bluetongue virus genome

R. E. Unger, R. Y. Chuang, L. F. Chuang, Bennie Osburn, R. H. Dol

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Bluetongue virus (BTV) total genomic and isolated individual segment dsRNAs end-labeled with 32P were successfully used as probes in colony hybridization to detect clones of BTV genomic material. The RNA probes were highly specific for cloned BTV genomic material. DNA probes, however, gave false positive results. DNA from bacterial clones was fixed to nylon and nitrocellulose membranes. The hybridized nylon membranes could be stripped of probe and reprobed at least 6 times without loss of signal strength.

Original languageEnglish (US)
Pages (from-to)251-263
Number of pages13
JournalJournal of Virological Methods
Volume20
Issue number3
DOIs
StatePublished - Jan 1 1988

Keywords

  • Bluetongue virus
  • Colony hybridization
  • Nylon membrane
  • RNA probe

ASJC Scopus subject areas

  • Virology

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