Colocalized delivery of adjuvant and antigen using nanolipoprotein particles enhances the immune response to recombinant antigens

Nicholas O Fischer, Amy Rasley, Michele Corzett, Mona H. Hwang, Paul D. Hoeprich, Craig D. Blanchette

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

Subunit antigen-based vaccines can provide a number of important benefits over traditional vaccine candidates, such as overall safety. However, because of the inherently low immunogenicity of these antigens, methods for colocalized delivery of antigen and immunostimulatory molecules (i.e., adjuvants) are needed. Here we report a robust nanolipoprotein particle (NLP)-based vaccine delivery platform that facilitates the codelivery of both subunit antigens and adjuvants. Ni-chelating NLPs (NiNLPs) were assembled to incorporate the amphipathic adjuvants monophosphoryl lipid A and cholesterol-modified CpG oligodeoxynucleotides, which can bind His-tagged protein antigens. Colocalization of antigen and adjuvant delivery using the NiNLP platform resulted in elevated antibody production against His-tagged influenza hemagglutinin 5 and Yersinia pestis LcrV antigens. Antibody titers in mice immunized with the adjuvanted NLPs were 5-10 times higher than those observed with coadministration formulations and nonadjuvanted NiNLPs. Colocalized delivery of adjuvant and antigen provides significantly greater immune stimulation in mice than coadministered formulations.

Original languageEnglish (US)
Pages (from-to)2044-2047
Number of pages4
JournalJournal of the American Chemical Society
Volume135
Issue number6
DOIs
StatePublished - Feb 13 2013
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Fingerprint Dive into the research topics of 'Colocalized delivery of adjuvant and antigen using nanolipoprotein particles enhances the immune response to recombinant antigens'. Together they form a unique fingerprint.

  • Cite this