Collisions between yeast chromosomal loci in vivo are governed by three layers of organization

Sean M. Burgess, Nancy Kleckner

Research output: Contribution to journalArticle

73 Scopus citations

Abstract

The relative probabilities that different pairs of chromosomal loci will collide with one another in vegetatively growing diploid yeast cells have been assessed using a genetic assay for Cre/loxP site-specific recombination. Recombination rates have been determined for 18 different pairs of loxP sites representing diverse pairs of positions within the genome. Overall, relative collision probabilities vary over an eightfold range. Within this range, a hierarchy comprising three levels of organization can be discerned. First, collisions between loci on nonhomologous chromosomes are governed by nonspecific centromere clustering. Second, a sequence is closer to allelic or nearby sequences on its homolog than to sequences on nonhomologous chromosomes, an effect most simply attributed to homolog pairing. Third, a sequence can be closer to other sequences nearby on the same chromosome than to sequences on other chromosomes. These findings provide a framework for assessing the role of chromosome disposition in cellular processes such as DNA repair and gene expression. Also the possibility is raised that genome- wide coalignment of homologs is not the fundamental raison d'etre of the somatic pairing process. We suggest instead that pairing may exist to promote juxtaposition of homologous regions within irregular genome complements.

Original languageEnglish (US)
Pages (from-to)1871-1883
Number of pages13
JournalGenes and Development
Volume13
Issue number14
StatePublished - Jul 15 1999

Keywords

  • Chromosome
  • DNA repair
  • Homolog pairing
  • Nucleus
  • Saccharomyces cerevisiae
  • Site-specific recombination

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Fingerprint Dive into the research topics of 'Collisions between yeast chromosomal loci in vivo are governed by three layers of organization'. Together they form a unique fingerprint.

  • Cite this