Collision-Induced Dissociation of Branched Oligosaccharide Ions with Analysis and Calculation of Relative Dissociation Thresholds

Sharron G. Penn, Mark T. Cancilla, Carlito B Lebrilla

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85 Scopus citations

Abstract

Collision-induced dissociation (CID) is used in an external source Fourier transform mass spectrometer (FTMS) equipped with matrix-assisted laser desorption/ionization (MALDI) to study a number of complex, branched oligosaccharides. The relative dissociation thresholds for various oligosaccharide fragmentation pathways have been calculated in terms of kinetic and center-of-mass frame energy. For two isomers of difucosyllacto-N-hexaose, the loss of the fucose sugar is always the lowest energy fragment observed and occurs at the same energy for both isomers when the oligosaccharide is coordinated to a sodium ion. When the oligosaccharide is complexed to cesium, the threshold for the removal of the fucose moiety increases, indicating that the cesium is involved in a coordination complex that stabilizes the sugar. MS/MS/MS is performed on a sugar, mannose core, which does not readily fragment during MALDI. In all the sugars examined, CID produces additional structural information relative to MALDI/FTMS.

Original languageEnglish (US)
Pages (from-to)2331-2339
Number of pages9
JournalAnalytical Chemistry
Volume68
Issue number14
StatePublished - Jul 15 1996

ASJC Scopus subject areas

  • Analytical Chemistry

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