Collection of Arabidopsis thaliana Morphological Insertion Mutants

O. A. Ogarkova, N. B. Tomilova, Alexey Tomilov, V. A. Tarasov

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A collection of transgenic Arabidopsis thaliana plants has been obtained by Agrobacterium-mediated transformation. The genomes of the transgenic plants contain insertions of T-DNA of the vector plasmids pLD3 or pPCVRN4. Genes bearing T-DNA insertions were shown to constitute 12-18% of the total number of A. thaliana genes. Seventy-five lines have been chosen from the collection and subjected to genetic and molecular-genetic analysis. Of these, 5 were dominant mutants, and 70, recessive insertion mutants with various morphological defects. Identification of mutant phenotypes and genetic characterization of the transgenic lines have been performed with the use of nutrient media supplemented with exogenous hormones, which revealed five recessive lethal mutants and one dominant sterile mutant.

Original languageEnglish (US)
Pages (from-to)899-904
Number of pages6
JournalRussian Journal of Genetics
Volume37
Issue number8
DOIs
StatePublished - Aug 1 2001
Externally publishedYes

Fingerprint

Arabidopsis
Agrobacterium
Genetically Modified Plants
Genes
Molecular Biology
Plasmids
Genome
Hormones
Phenotype
Food
T-DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Collection of Arabidopsis thaliana Morphological Insertion Mutants. / Ogarkova, O. A.; Tomilova, N. B.; Tomilov, Alexey; Tarasov, V. A.

In: Russian Journal of Genetics, Vol. 37, No. 8, 01.08.2001, p. 899-904.

Research output: Contribution to journalArticle

Ogarkova, O. A. ; Tomilova, N. B. ; Tomilov, Alexey ; Tarasov, V. A. / Collection of Arabidopsis thaliana Morphological Insertion Mutants. In: Russian Journal of Genetics. 2001 ; Vol. 37, No. 8. pp. 899-904.
@article{7223837a62f6488ab195d34603a76b21,
title = "Collection of Arabidopsis thaliana Morphological Insertion Mutants",
abstract = "A collection of transgenic Arabidopsis thaliana plants has been obtained by Agrobacterium-mediated transformation. The genomes of the transgenic plants contain insertions of T-DNA of the vector plasmids pLD3 or pPCVRN4. Genes bearing T-DNA insertions were shown to constitute 12-18{\%} of the total number of A. thaliana genes. Seventy-five lines have been chosen from the collection and subjected to genetic and molecular-genetic analysis. Of these, 5 were dominant mutants, and 70, recessive insertion mutants with various morphological defects. Identification of mutant phenotypes and genetic characterization of the transgenic lines have been performed with the use of nutrient media supplemented with exogenous hormones, which revealed five recessive lethal mutants and one dominant sterile mutant.",
author = "Ogarkova, {O. A.} and Tomilova, {N. B.} and Alexey Tomilov and Tarasov, {V. A.}",
year = "2001",
month = "8",
day = "1",
doi = "10.1023/A:1016725632466",
language = "English (US)",
volume = "37",
pages = "899--904",
journal = "Russian Journal of Genetics",
issn = "1022-7954",
publisher = "Maik Nauka-Interperiodica Publishing",
number = "8",

}

TY - JOUR

T1 - Collection of Arabidopsis thaliana Morphological Insertion Mutants

AU - Ogarkova, O. A.

AU - Tomilova, N. B.

AU - Tomilov, Alexey

AU - Tarasov, V. A.

PY - 2001/8/1

Y1 - 2001/8/1

N2 - A collection of transgenic Arabidopsis thaliana plants has been obtained by Agrobacterium-mediated transformation. The genomes of the transgenic plants contain insertions of T-DNA of the vector plasmids pLD3 or pPCVRN4. Genes bearing T-DNA insertions were shown to constitute 12-18% of the total number of A. thaliana genes. Seventy-five lines have been chosen from the collection and subjected to genetic and molecular-genetic analysis. Of these, 5 were dominant mutants, and 70, recessive insertion mutants with various morphological defects. Identification of mutant phenotypes and genetic characterization of the transgenic lines have been performed with the use of nutrient media supplemented with exogenous hormones, which revealed five recessive lethal mutants and one dominant sterile mutant.

AB - A collection of transgenic Arabidopsis thaliana plants has been obtained by Agrobacterium-mediated transformation. The genomes of the transgenic plants contain insertions of T-DNA of the vector plasmids pLD3 or pPCVRN4. Genes bearing T-DNA insertions were shown to constitute 12-18% of the total number of A. thaliana genes. Seventy-five lines have been chosen from the collection and subjected to genetic and molecular-genetic analysis. Of these, 5 were dominant mutants, and 70, recessive insertion mutants with various morphological defects. Identification of mutant phenotypes and genetic characterization of the transgenic lines have been performed with the use of nutrient media supplemented with exogenous hormones, which revealed five recessive lethal mutants and one dominant sterile mutant.

UR - http://www.scopus.com/inward/record.url?scp=0141919298&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141919298&partnerID=8YFLogxK

U2 - 10.1023/A:1016725632466

DO - 10.1023/A:1016725632466

M3 - Article

AN - SCOPUS:0141919298

VL - 37

SP - 899

EP - 904

JO - Russian Journal of Genetics

JF - Russian Journal of Genetics

SN - 1022-7954

IS - 8

ER -