Collection of arabidopsis thaliana morphological insertion mutants

O. A. Ogarkova, N. B. Tomilova, Alexey Tomilov, V. A. Tarasov

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

A collection of transgenic Arabidopsis thaliana plants has been obtained by Agrobacterium-mediated transformation. The genomes of the transgenic plants contain insertions of T-DNA of the vector plasmids pLD3 or pPCVRN4. Genes bearing T-DNA insertions were shown to constitute 12-18% of the total number of A. thaliana genes. Seventy-five lines have been chosen from the collection and subjected to genetic and molecular-genetic analysis. Of these, 5 were dominant mutants, and 70, recessive insertion mutants with various morphological defects. Identification of mutant phenotypes and genetic characterization of the transgenic lines have been performed with the use of nutrient media supplemented with exogenous hormones, which revealed five recessive lethal mutants and one dominant sterile mutant.

Original languageEnglish (US)
Pages (from-to)1081-1087
Number of pages7
JournalGenetika
Volume37
Issue number8
StatePublished - Dec 1 2001
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'Collection of arabidopsis thaliana morphological insertion mutants'. Together they form a unique fingerprint.

  • Cite this

    Ogarkova, O. A., Tomilova, N. B., Tomilov, A., & Tarasov, V. A. (2001). Collection of arabidopsis thaliana morphological insertion mutants. Genetika, 37(8), 1081-1087.