Collagen fiber organization is related to mechanical properties and remodeling in equine bone. A comparsion of two methods

R. B. Martin, S. T. Lau, P. V. Mathews, V. A. Gibson, Susan M Stover

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

We studied birefringence as an indicator of collagen fiber orientation in the diaphysis of the equine third metacarpal bone. We had previously shown that tissue from the lateral cortex of this bone is stronger monotonically, but less fatigue resistant, than tissue from the medial and dorsal regions. To learn whether collagen fiber orientation might play a role in this regional specialization, we tested three hypotheses using the same specimens: (1) collagen fiber orientation is regionally dependent; (2) remodeling changes collagen fiber orientation; (3) longitudinal collagen fibers correlate positively with modulus and monotonic bending strength and negatively with flexural fatigue life. Beams (N = 36) cut parallel to the long axes of six pairs of bones had been tested to determine elastic modulus (N = 36), and fatigue life (N = 24) or monotonic strength (N = 12) in four-point bending. Subsequently, histologic cross-sections were prepared, and porosity, active remodeling and past remodeling were quantified. Birefringence was measured as an indicator of transverse collagen orientation using plane-polarized light (PPL), and again using circularly polarized light (CPL). The CPL measurement was less variable than the PPL measurement. Both birefringence measures indicated that collagen was more longitudinally oriented in the lateral cortex than in the other two cortices. Longitudinally disposed collagen correlated with greater modulus and monotonic strength, but did not correlate with fatigue life. Remodeling was associated with more transverse collagen. Neither measure of birefringence was significantly correlated with porosity. It was concluded that, in the equine cannon bone, longitudinal collagen fiber orientation is regionally variable, contributes to increased modulus and strength but not fatigue life, and is reduced by osteonal remodeling.

Original languageEnglish (US)
Pages (from-to)1515-1521
Number of pages7
JournalJournal of Biomechanics
Volume29
Issue number12
DOIs
StatePublished - Dec 1996

Fingerprint

Collagen
Horses
Bone
Bone and Bones
Mechanical properties
Fibers
Birefringence
Fiber reinforced materials
Fatigue
Light polarization
Fatigue of materials
Light measurement
Light
Porosity
Tissue
Diaphyses
Metacarpal Bones
Elastic Modulus
Bending strength
Elastic moduli

Keywords

  • Birefringence
  • Bone
  • Collagen fibers
  • Lamellae
  • Mechanial properties

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Collagen fiber organization is related to mechanical properties and remodeling in equine bone. A comparsion of two methods. / Martin, R. B.; Lau, S. T.; Mathews, P. V.; Gibson, V. A.; Stover, Susan M.

In: Journal of Biomechanics, Vol. 29, No. 12, 12.1996, p. 1515-1521.

Research output: Contribution to journalArticle

Martin, R. B. ; Lau, S. T. ; Mathews, P. V. ; Gibson, V. A. ; Stover, Susan M. / Collagen fiber organization is related to mechanical properties and remodeling in equine bone. A comparsion of two methods. In: Journal of Biomechanics. 1996 ; Vol. 29, No. 12. pp. 1515-1521.
@article{500099d3149d414f82aed0ab944ef6a2,
title = "Collagen fiber organization is related to mechanical properties and remodeling in equine bone. A comparsion of two methods",
abstract = "We studied birefringence as an indicator of collagen fiber orientation in the diaphysis of the equine third metacarpal bone. We had previously shown that tissue from the lateral cortex of this bone is stronger monotonically, but less fatigue resistant, than tissue from the medial and dorsal regions. To learn whether collagen fiber orientation might play a role in this regional specialization, we tested three hypotheses using the same specimens: (1) collagen fiber orientation is regionally dependent; (2) remodeling changes collagen fiber orientation; (3) longitudinal collagen fibers correlate positively with modulus and monotonic bending strength and negatively with flexural fatigue life. Beams (N = 36) cut parallel to the long axes of six pairs of bones had been tested to determine elastic modulus (N = 36), and fatigue life (N = 24) or monotonic strength (N = 12) in four-point bending. Subsequently, histologic cross-sections were prepared, and porosity, active remodeling and past remodeling were quantified. Birefringence was measured as an indicator of transverse collagen orientation using plane-polarized light (PPL), and again using circularly polarized light (CPL). The CPL measurement was less variable than the PPL measurement. Both birefringence measures indicated that collagen was more longitudinally oriented in the lateral cortex than in the other two cortices. Longitudinally disposed collagen correlated with greater modulus and monotonic strength, but did not correlate with fatigue life. Remodeling was associated with more transverse collagen. Neither measure of birefringence was significantly correlated with porosity. It was concluded that, in the equine cannon bone, longitudinal collagen fiber orientation is regionally variable, contributes to increased modulus and strength but not fatigue life, and is reduced by osteonal remodeling.",
keywords = "Birefringence, Bone, Collagen fibers, Lamellae, Mechanial properties",
author = "Martin, {R. B.} and Lau, {S. T.} and Mathews, {P. V.} and Gibson, {V. A.} and Stover, {Susan M}",
year = "1996",
month = "12",
doi = "10.1016/0021-9290(96)00082-6",
language = "English (US)",
volume = "29",
pages = "1515--1521",
journal = "Journal of Biomechanics",
issn = "0021-9290",
publisher = "Elsevier Limited",
number = "12",

}

TY - JOUR

T1 - Collagen fiber organization is related to mechanical properties and remodeling in equine bone. A comparsion of two methods

AU - Martin, R. B.

AU - Lau, S. T.

AU - Mathews, P. V.

AU - Gibson, V. A.

AU - Stover, Susan M

PY - 1996/12

Y1 - 1996/12

N2 - We studied birefringence as an indicator of collagen fiber orientation in the diaphysis of the equine third metacarpal bone. We had previously shown that tissue from the lateral cortex of this bone is stronger monotonically, but less fatigue resistant, than tissue from the medial and dorsal regions. To learn whether collagen fiber orientation might play a role in this regional specialization, we tested three hypotheses using the same specimens: (1) collagen fiber orientation is regionally dependent; (2) remodeling changes collagen fiber orientation; (3) longitudinal collagen fibers correlate positively with modulus and monotonic bending strength and negatively with flexural fatigue life. Beams (N = 36) cut parallel to the long axes of six pairs of bones had been tested to determine elastic modulus (N = 36), and fatigue life (N = 24) or monotonic strength (N = 12) in four-point bending. Subsequently, histologic cross-sections were prepared, and porosity, active remodeling and past remodeling were quantified. Birefringence was measured as an indicator of transverse collagen orientation using plane-polarized light (PPL), and again using circularly polarized light (CPL). The CPL measurement was less variable than the PPL measurement. Both birefringence measures indicated that collagen was more longitudinally oriented in the lateral cortex than in the other two cortices. Longitudinally disposed collagen correlated with greater modulus and monotonic strength, but did not correlate with fatigue life. Remodeling was associated with more transverse collagen. Neither measure of birefringence was significantly correlated with porosity. It was concluded that, in the equine cannon bone, longitudinal collagen fiber orientation is regionally variable, contributes to increased modulus and strength but not fatigue life, and is reduced by osteonal remodeling.

AB - We studied birefringence as an indicator of collagen fiber orientation in the diaphysis of the equine third metacarpal bone. We had previously shown that tissue from the lateral cortex of this bone is stronger monotonically, but less fatigue resistant, than tissue from the medial and dorsal regions. To learn whether collagen fiber orientation might play a role in this regional specialization, we tested three hypotheses using the same specimens: (1) collagen fiber orientation is regionally dependent; (2) remodeling changes collagen fiber orientation; (3) longitudinal collagen fibers correlate positively with modulus and monotonic bending strength and negatively with flexural fatigue life. Beams (N = 36) cut parallel to the long axes of six pairs of bones had been tested to determine elastic modulus (N = 36), and fatigue life (N = 24) or monotonic strength (N = 12) in four-point bending. Subsequently, histologic cross-sections were prepared, and porosity, active remodeling and past remodeling were quantified. Birefringence was measured as an indicator of transverse collagen orientation using plane-polarized light (PPL), and again using circularly polarized light (CPL). The CPL measurement was less variable than the PPL measurement. Both birefringence measures indicated that collagen was more longitudinally oriented in the lateral cortex than in the other two cortices. Longitudinally disposed collagen correlated with greater modulus and monotonic strength, but did not correlate with fatigue life. Remodeling was associated with more transverse collagen. Neither measure of birefringence was significantly correlated with porosity. It was concluded that, in the equine cannon bone, longitudinal collagen fiber orientation is regionally variable, contributes to increased modulus and strength but not fatigue life, and is reduced by osteonal remodeling.

KW - Birefringence

KW - Bone

KW - Collagen fibers

KW - Lamellae

KW - Mechanial properties

UR - http://www.scopus.com/inward/record.url?scp=0030560691&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030560691&partnerID=8YFLogxK

U2 - 10.1016/0021-9290(96)00082-6

DO - 10.1016/0021-9290(96)00082-6

M3 - Article

VL - 29

SP - 1515

EP - 1521

JO - Journal of Biomechanics

JF - Journal of Biomechanics

SN - 0021-9290

IS - 12

ER -