Abstract
We have studied the growth of neurites from single retinal ganglion cells isolated from adult goldfish and maintained under various primary cell culture conditions. In 10% Leibovitz's L-15 medium at 23°C, these ganglion cells remained viable for up to 10 days and generated extensive fields of neurites. We found two patterns of neuritic fields. In one, a pair of neurites exited from opposite sides of the cell soma, forming a bipolar pattern. In the second pattern, three to five neurites exited from several points around the soma, forming a multipolar pattern. Characteristically, each neurite of this latter type tapered and branched two to seven times, whereas neurites forming bipolar patterns showed less branching and little or no taper. The fields subtended by the neurites in multipolar patterns ranged in size from 33000 to 204000 μm2. Finally, although these neurites grew as fast as 35 μm hr-1 at 23°C and individually reached lengths of up to 735 μm, they showed essentially no growth at 13°C. Neurite outgrowth at 23°C was vigorous even in cells whose growth had previously been suppressed for as long as 8 hr at 13°C.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 175-191 |
| Number of pages | 17 |
| Journal | Experimental Eye Research |
| Volume | 52 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1991 |
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Keywords
- axons
- dendrites
- dissociated retinal cells
- neurites
- regeneration
- retinal ganglion cell
- temperature sensitivity
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
Cite this
Cold inhibits neurite outgrowth from single retinal ganglion cells isolated from adult goldfish. / Ishida, Andrew; Cheng, M. H.
In: Experimental Eye Research, Vol. 52, No. 2, 1991, p. 175-191.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cold inhibits neurite outgrowth from single retinal ganglion cells isolated from adult goldfish
AU - Ishida, Andrew
AU - Cheng, M. H.
PY - 1991
Y1 - 1991
N2 - We have studied the growth of neurites from single retinal ganglion cells isolated from adult goldfish and maintained under various primary cell culture conditions. In 10% Leibovitz's L-15 medium at 23°C, these ganglion cells remained viable for up to 10 days and generated extensive fields of neurites. We found two patterns of neuritic fields. In one, a pair of neurites exited from opposite sides of the cell soma, forming a bipolar pattern. In the second pattern, three to five neurites exited from several points around the soma, forming a multipolar pattern. Characteristically, each neurite of this latter type tapered and branched two to seven times, whereas neurites forming bipolar patterns showed less branching and little or no taper. The fields subtended by the neurites in multipolar patterns ranged in size from 33000 to 204000 μm2. Finally, although these neurites grew as fast as 35 μm hr-1 at 23°C and individually reached lengths of up to 735 μm, they showed essentially no growth at 13°C. Neurite outgrowth at 23°C was vigorous even in cells whose growth had previously been suppressed for as long as 8 hr at 13°C.
AB - We have studied the growth of neurites from single retinal ganglion cells isolated from adult goldfish and maintained under various primary cell culture conditions. In 10% Leibovitz's L-15 medium at 23°C, these ganglion cells remained viable for up to 10 days and generated extensive fields of neurites. We found two patterns of neuritic fields. In one, a pair of neurites exited from opposite sides of the cell soma, forming a bipolar pattern. In the second pattern, three to five neurites exited from several points around the soma, forming a multipolar pattern. Characteristically, each neurite of this latter type tapered and branched two to seven times, whereas neurites forming bipolar patterns showed less branching and little or no taper. The fields subtended by the neurites in multipolar patterns ranged in size from 33000 to 204000 μm2. Finally, although these neurites grew as fast as 35 μm hr-1 at 23°C and individually reached lengths of up to 735 μm, they showed essentially no growth at 13°C. Neurite outgrowth at 23°C was vigorous even in cells whose growth had previously been suppressed for as long as 8 hr at 13°C.
KW - axons
KW - dendrites
KW - dissociated retinal cells
KW - neurites
KW - regeneration
KW - retinal ganglion cell
KW - temperature sensitivity
UR - http://www.scopus.com/inward/record.url?scp=0025969428&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025969428&partnerID=8YFLogxK
U2 - 10.1016/0014-4835(91)90257-F
DO - 10.1016/0014-4835(91)90257-F
M3 - Article
C2 - 2013300
AN - SCOPUS:0025969428
VL - 52
SP - 175
EP - 191
JO - Experimental Eye Research
JF - Experimental Eye Research
SN - 0014-4835
IS - 2
ER -