Cointegrate formation by IS 50 requires multiple donor molecules

Ann Lichens-Park, Michael Syvanen

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The insertion sequence, IS 50R, promotes cointegrate formation between a lambda::IS 50R phage and the chromosome of Escherichia coli strain C. We show that formation of cointegrates mediated by IS 50R between the non-replicating phage genome and the bacterial chromosome requires multiple donor molecules and depends on homologous recombination functions. We conclude that the two copies of IS 50 present in the cointegrate originate in two different molecules. Thus, the existence of the cointegrate structure cannot be used as evidence for replication of IS 50 sequences during IS 50 transposition.

Original languageEnglish (US)
Pages (from-to)244-251
Number of pages8
JournalMGG Molecular & General Genetics
Volume211
Issue number2
DOIs
StatePublished - Feb 1988

Keywords

  • Bacteriophage lambda
  • Cointegrate
  • Dimer donor model
  • Insertion sequence
  • IS 50

ASJC Scopus subject areas

  • Genetics

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