Coinfection with Bartonella clarridgeiae and Bartonella henselae and with different Bartonella henselae strains in domestic cats

A. Nikos Gurfield, Henri Jean Boulouis, Bruno B Chomel, Rémy Heller, Rickie W. Kasten, Kazuhiro Yamamoto, Yves Piemont

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Bartonella clarridgeiae and several strains of Bartonella henselae, the agent of cat scratch disease, with variations in the 16S rRNA gene have been found to infect the blood of cats. An epidemiologic study of Bartonella infection in domestic French cats revealed that of 436 cats sampled, 5 cats (1.1%) were coinfected with B. henselae and B. clarridgeiae and 2 cats (0.5%) were coinfected with two strains of B. henselae with variations in the 16S rRNA gene, B. henselae type I and type II. In an indirect immunofluorescence assay, coinfected cats tested positive for both Bartonella species at titers of ≤128. Identification of the colonies was achieved by preformed enzyme analysis, PCR-restriction fragment length polymorphism analysis of the citrate synthase gene, and 16S rRNA gene sequencing. Colony size differences in mixed culture allowed differentiation of the Bartonella species. The coinfection of cats with two Bartonella species or variants of the same species raises concern about the possibility of dual infection in humans. The development of a polyvalent vaccine targeted against the most pathogenic or invasive strains may be a means of protecting cats and man from infection.

Original languageEnglish (US)
Pages (from-to)2120-2123
Number of pages4
JournalJournal of Clinical Microbiology
Volume35
Issue number8
StatePublished - Aug 1997

Fingerprint

Bartonella henselae
Bartonella
Coinfection
Cats
rRNA Genes
Bartonella Infections
Cat-Scratch Disease
Citrate (si)-Synthase
Indirect Fluorescent Antibody Technique
Infection
Restriction Fragment Length Polymorphisms
Epidemiologic Studies
Vaccines
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Gurfield, A. N., Boulouis, H. J., Chomel, B. B., Heller, R., Kasten, R. W., Yamamoto, K., & Piemont, Y. (1997). Coinfection with Bartonella clarridgeiae and Bartonella henselae and with different Bartonella henselae strains in domestic cats. Journal of Clinical Microbiology, 35(8), 2120-2123.

Coinfection with Bartonella clarridgeiae and Bartonella henselae and with different Bartonella henselae strains in domestic cats. / Gurfield, A. Nikos; Boulouis, Henri Jean; Chomel, Bruno B; Heller, Rémy; Kasten, Rickie W.; Yamamoto, Kazuhiro; Piemont, Yves.

In: Journal of Clinical Microbiology, Vol. 35, No. 8, 08.1997, p. 2120-2123.

Research output: Contribution to journalArticle

Gurfield, AN, Boulouis, HJ, Chomel, BB, Heller, R, Kasten, RW, Yamamoto, K & Piemont, Y 1997, 'Coinfection with Bartonella clarridgeiae and Bartonella henselae and with different Bartonella henselae strains in domestic cats', Journal of Clinical Microbiology, vol. 35, no. 8, pp. 2120-2123.
Gurfield, A. Nikos ; Boulouis, Henri Jean ; Chomel, Bruno B ; Heller, Rémy ; Kasten, Rickie W. ; Yamamoto, Kazuhiro ; Piemont, Yves. / Coinfection with Bartonella clarridgeiae and Bartonella henselae and with different Bartonella henselae strains in domestic cats. In: Journal of Clinical Microbiology. 1997 ; Vol. 35, No. 8. pp. 2120-2123.
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