Coinfection with Anaplasma phagocytophilum alters Borrelia burgdorferi population distribution in C3H/HeN mice

Kevin Holden, Emir Hodzic, Sunlian Feng, Kimberly J. Freet, Rance B Lefebvre, Stephen W Barthold

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Borrelia burgdorferi, the agent of Lyme disease, and Anaplasma phagocytophilum, the agent of human anaplasmosis, are both transmitted by Ixodes sp. ticks and may occasionally coinfect a host. The population distributions of tick-transmitted B. burgdorferi infection were assessed using quantitative PCR targeting the flaB gene of B. burgdorferi in the ear, heart base, quadriceps muscle, skin, and tibiotarsal joint tissue of C3H mice previously infected with A. phagocytophilum. Population distributions of Anaplasma infection were assessed by targeting the p44 gene. A. phagocytophilum in blood and serologic response to both agents were evaluated. Spirochete numbers were increased in the ears, heart base, and skin of coinfected mice, but Anaplasma numbers remained constant. Antibody response to A. phagocytophilum, but not B. burgdorferi, was decreased in coinfected mice. These results suggest that coinfection with A. phagocytophilum and B. burgdorferi modulates pathogen burden and host antibody responses. This may be explained by the ability of A. phagocytophilum to functionally impair neutrophils, important cells in the early defense against B. burgdorferi infection.

Original languageEnglish (US)
Pages (from-to)3440-3444
Number of pages5
JournalInfection and Immunity
Volume73
Issue number6
DOIs
StatePublished - Jun 2005

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Anaplasma phagocytophilum
Borrelia burgdorferi
Inbred C3H Mouse
Coinfection
Demography
Anaplasma
Borrelia Infections
Gene Targeting
Ticks
Antibody Formation
Ear
Anaplasmosis
Ixodes
Spirochaetales
Skin
Lyme Disease
Quadriceps Muscle
Neutrophils
Joints
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Immunology

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Coinfection with Anaplasma phagocytophilum alters Borrelia burgdorferi population distribution in C3H/HeN mice. / Holden, Kevin; Hodzic, Emir; Feng, Sunlian; Freet, Kimberly J.; Lefebvre, Rance B; Barthold, Stephen W.

In: Infection and Immunity, Vol. 73, No. 6, 06.2005, p. 3440-3444.

Research output: Contribution to journalArticle

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