Coincident activation of Th2 T cells with onset of the disease and differential expression of GRO-gamma in peripheral blood leukocytes in minimal change disease

Horacio E. Adrogue, Jason Borillo, Lisa Torres, Arundhati S Kale, Cindy Zhou, Daniel Feig, Justin Merszei, Richard Johnson, Ya Huan Lou

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: Involvement of Th2 T cells/NFκB in minimal change disease (MCD) has been postulated. A promising but unconfirmed glomerular permeability factor (GPF) from MCD T cells has been described. We explored whether GPF was the consequence of Th2 cell activation. Methods: Peripheral blood leukocytes (PBL) from 16 MCD patients and 7 normal controls were analyzed and the results were statistically compared. Results: Flow cytometry demonstrated a significant expansion of CD4+ T cell population and dramatically increased CD69+ cells among CD4+ T cells in MCD, suggesting coincident activation of T cells with onset of the disease. RT-PCR on RNA from either freshly isolated PBL or post in vitroactivation showed high-level expression of the Th2 cytokine interleukin-4 in all MCD patients. Importantly, both antibody microarray assay on sera and RT-PCR on mRNA of PBL revealed expression of a CXC chemokine GRO-γ (growth-related oncogene) in all MCD patients as compared with one of 7 controls. Conclusions: Our results reveal an association between onset of MCD and activation of Th2 cells. The GRO family has been implicated in the function of endothelial cells, and its expression is under NFκB regulation. Thus, GRO-γ is a promising candidate for Th2-associated GPF in MCD.

Original languageEnglish (US)
Pages (from-to)253-261
Number of pages9
JournalAmerican Journal of Nephrology
Volume27
Issue number3
DOIs
StatePublished - May 1 2007
Externally publishedYes

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Lipoid Nephrosis
Th2 Cells
Leukocytes
T-Lymphocytes
Permeability
CXC Chemokines
Polymerase Chain Reaction
Oncogenes
Interleukin-4
Flow Cytometry
Endothelial Cells
RNA
Cytokines
Messenger RNA
Antibodies

Keywords

  • Chemokine
  • Growth-related oncogene-γ
  • Minimal change disease
  • Th2 T cell activation

ASJC Scopus subject areas

  • Nephrology

Cite this

Coincident activation of Th2 T cells with onset of the disease and differential expression of GRO-gamma in peripheral blood leukocytes in minimal change disease. / Adrogue, Horacio E.; Borillo, Jason; Torres, Lisa; Kale, Arundhati S; Zhou, Cindy; Feig, Daniel; Merszei, Justin; Johnson, Richard; Lou, Ya Huan.

In: American Journal of Nephrology, Vol. 27, No. 3, 01.05.2007, p. 253-261.

Research output: Contribution to journalArticle

Adrogue, Horacio E. ; Borillo, Jason ; Torres, Lisa ; Kale, Arundhati S ; Zhou, Cindy ; Feig, Daniel ; Merszei, Justin ; Johnson, Richard ; Lou, Ya Huan. / Coincident activation of Th2 T cells with onset of the disease and differential expression of GRO-gamma in peripheral blood leukocytes in minimal change disease. In: American Journal of Nephrology. 2007 ; Vol. 27, No. 3. pp. 253-261.
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