Coherence-Gated Sensorless Adaptive Optics Multiphoton Retinal Imaging

Michelle Cua, Daniel J. Wahl, Yuan Zhao, Sujin Lee, Stefano Bonora, Robert Zawadzki, Yifan Jian, Marinko V. Sarunic

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Multiphoton microscopy enables imaging deep into scattering tissues. The efficient generation of non-linear optical effects is related to both the pulse duration (typically on the order of femtoseconds) and the size of the focused spot. Aberrations introduced by refractive index inhomogeneity in the sample distort the wavefront and enlarge the focal spot, which reduces the multiphoton signal. Traditional approaches to adaptive optics wavefront correction are not effective in thick or multi-layered scattering media. In this report, we present sensorless adaptive optics (SAO) using low-coherence interferometric detection of the excitation light for depth-resolved aberration correction of two-photon excited fluorescence (TPEF) in biological tissue. We demonstrate coherence-gated SAO TPEF using a transmissive multi-actuator adaptive lens for in vivo imaging in a mouse retina. This configuration has significant potential for reducing the laser power required for adaptive optics multiphoton imaging, and for facilitating integration with existing systems.

Original languageEnglish (US)
Article number32223
JournalScientific Reports
Volume6
DOIs
StatePublished - Sep 7 2016

ASJC Scopus subject areas

  • General

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    Cua, M., Wahl, D. J., Zhao, Y., Lee, S., Bonora, S., Zawadzki, R., Jian, Y., & Sarunic, M. V. (2016). Coherence-Gated Sensorless Adaptive Optics Multiphoton Retinal Imaging. Scientific Reports, 6, [32223]. https://doi.org/10.1038/srep32223