Cognitive dysfunction associated with diabetic ketoacidosis in rats

Nicole Glaser, Steve Anderson, Wesley Leong, Daniel J Tancredi, Martha E O'Donnell

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Background: Type 1 diabetes mellitus in children may be associated with neurocognitive deficits of unclear cause. A recent retrospective study in children suggested possible associations between diabetic ketoacidosis (DKA) and decreased memory. The current investigation was undertaken to determine whether cognitive deficits could be detected after a single episode of DKA in an animal model. Methods: Streptozotocin was used to induce diabetes in juvenile rats, and rats were then treated with subcutaneous insulin injections. In one group, insulin was subsequently withdrawn to allow development of DKA, which was then treated with insulin and saline. After recovery from DKA, subcutaneous insulin injections were re-started. In the diabetes control group, rats continued to receive subcutaneous insulin and underwent sham procedures identical to the DKA group. One week after recovery, cognitive function was tested using the Morris Water Maze, a procedure that requires rats to locate a hidden platform in a water pool using visual cues. During a 10 day period, mean time to locate the platform (latency) during 4 trials per day was recorded. Results: Comparison of latency curves demonstrated longer mean latency times on days 7 and 8 in the DKA group indicating delayed learning compared to diabetic controls. Conclusions: These data demonstrate that a single DKA episode results in measurable deficits in learning in rats, consistent with findings that DKA may contribute to neurocognitive deficits in children with type 1 diabetes.

Original languageEnglish (US)
Pages (from-to)110-114
Number of pages5
JournalNeuroscience Letters
Issue number2
StatePublished - Feb 29 2012


  • Brain injury
  • Diabetic ketoacidosis
  • Neurocognitive
  • Pediatric

ASJC Scopus subject areas

  • Neuroscience(all)


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