Cofactor symbiosis for enhanced algal growth, biofuel production, and wastewater treatment

Brendan T. Higgins, Ingrid Gennity, Stephanie Samra, Tobias Kind, Oliver Fiehn, Jean S. VanderGheynst

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Algae have gained attention for production of fuels and chemicals, and treatment of wastewater. The high cost of algae cultivation, however, has limited industry adoption for these applications. Developing methods to increase algal growth rates and lipid content has emerged as an important strategy toward reducing production costs, and significant research effort has been exerted in this area. We have reported previously that co-culturing the green alga, Auxenochlorella protothecoides, with Escherichia coli under mixotrophic conditions led to 2-6 fold increases in algal growth, doubling of neutral lipid content, and elevated nutrient removal rates compared to axenic growth, indicative of a symbiotic relationship. In the present work, we reveal that symbiosis stems largely from E. coli's provision of thiamine derivatives and degradation products to A. protothecoides. LCMS showed that residual cell-free medium obtained from axenic E. coli culture contained roughly 1.15 nM thiamine pyrophosphate and 4.0-9.1 nM of the thiamine precursor and degradation product, 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP). These compounds were found to promote the growth, lipid content, and glucose uptake of A. protothecoides, while dramatically improving substrate utilization efficiency. Due to widespread cofactor auxotrophy among algae, the co-culture results presented here likely extend to a large number of microbial community systems. We show that algal-algal symbiosis based on cofactor exchange is also possible, opening a new frontier in algae cultivation management. These findings highlight the potential of engineered microbial communities for improved algal biofuel production and wastewater treatment.

Original languageEnglish (US)
Pages (from-to)308-315
Number of pages8
JournalAlgal Research
Volume17
DOIs
StatePublished - Jul 1 2016

Fingerprint

algae
wastewater treatment
biofuels
symbiosis
thiamin
lipid content
Escherichia coli
microbial communities
fuel production
degradation
pyrophosphates
coculture
production costs
Chlorophyta
chemical derivatives
triacylglycerols
industry
uptake mechanisms
glucose
stems

Keywords

  • Biofuel
  • Co-culture
  • Cofactor
  • Organism interaction
  • Symbiosis
  • Wastewater treatment

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Higgins, B. T., Gennity, I., Samra, S., Kind, T., Fiehn, O., & VanderGheynst, J. S. (2016). Cofactor symbiosis for enhanced algal growth, biofuel production, and wastewater treatment. Algal Research, 17, 308-315. https://doi.org/10.1016/j.algal.2016.05.024

Cofactor symbiosis for enhanced algal growth, biofuel production, and wastewater treatment. / Higgins, Brendan T.; Gennity, Ingrid; Samra, Stephanie; Kind, Tobias; Fiehn, Oliver; VanderGheynst, Jean S.

In: Algal Research, Vol. 17, 01.07.2016, p. 308-315.

Research output: Contribution to journalArticle

Higgins, BT, Gennity, I, Samra, S, Kind, T, Fiehn, O & VanderGheynst, JS 2016, 'Cofactor symbiosis for enhanced algal growth, biofuel production, and wastewater treatment', Algal Research, vol. 17, pp. 308-315. https://doi.org/10.1016/j.algal.2016.05.024
Higgins, Brendan T. ; Gennity, Ingrid ; Samra, Stephanie ; Kind, Tobias ; Fiehn, Oliver ; VanderGheynst, Jean S. / Cofactor symbiosis for enhanced algal growth, biofuel production, and wastewater treatment. In: Algal Research. 2016 ; Vol. 17. pp. 308-315.
@article{d0b3ab51f26e4f34853d44c398e225d3,
title = "Cofactor symbiosis for enhanced algal growth, biofuel production, and wastewater treatment",
abstract = "Algae have gained attention for production of fuels and chemicals, and treatment of wastewater. The high cost of algae cultivation, however, has limited industry adoption for these applications. Developing methods to increase algal growth rates and lipid content has emerged as an important strategy toward reducing production costs, and significant research effort has been exerted in this area. We have reported previously that co-culturing the green alga, Auxenochlorella protothecoides, with Escherichia coli under mixotrophic conditions led to 2-6 fold increases in algal growth, doubling of neutral lipid content, and elevated nutrient removal rates compared to axenic growth, indicative of a symbiotic relationship. In the present work, we reveal that symbiosis stems largely from E. coli's provision of thiamine derivatives and degradation products to A. protothecoides. LCMS showed that residual cell-free medium obtained from axenic E. coli culture contained roughly 1.15 nM thiamine pyrophosphate and 4.0-9.1 nM of the thiamine precursor and degradation product, 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP). These compounds were found to promote the growth, lipid content, and glucose uptake of A. protothecoides, while dramatically improving substrate utilization efficiency. Due to widespread cofactor auxotrophy among algae, the co-culture results presented here likely extend to a large number of microbial community systems. We show that algal-algal symbiosis based on cofactor exchange is also possible, opening a new frontier in algae cultivation management. These findings highlight the potential of engineered microbial communities for improved algal biofuel production and wastewater treatment.",
keywords = "Biofuel, Co-culture, Cofactor, Organism interaction, Symbiosis, Wastewater treatment",
author = "Higgins, {Brendan T.} and Ingrid Gennity and Stephanie Samra and Tobias Kind and Oliver Fiehn and VanderGheynst, {Jean S.}",
year = "2016",
month = "7",
day = "1",
doi = "10.1016/j.algal.2016.05.024",
language = "English (US)",
volume = "17",
pages = "308--315",
journal = "Algal Research",
issn = "2211-9264",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Cofactor symbiosis for enhanced algal growth, biofuel production, and wastewater treatment

AU - Higgins, Brendan T.

AU - Gennity, Ingrid

AU - Samra, Stephanie

AU - Kind, Tobias

AU - Fiehn, Oliver

AU - VanderGheynst, Jean S.

PY - 2016/7/1

Y1 - 2016/7/1

N2 - Algae have gained attention for production of fuels and chemicals, and treatment of wastewater. The high cost of algae cultivation, however, has limited industry adoption for these applications. Developing methods to increase algal growth rates and lipid content has emerged as an important strategy toward reducing production costs, and significant research effort has been exerted in this area. We have reported previously that co-culturing the green alga, Auxenochlorella protothecoides, with Escherichia coli under mixotrophic conditions led to 2-6 fold increases in algal growth, doubling of neutral lipid content, and elevated nutrient removal rates compared to axenic growth, indicative of a symbiotic relationship. In the present work, we reveal that symbiosis stems largely from E. coli's provision of thiamine derivatives and degradation products to A. protothecoides. LCMS showed that residual cell-free medium obtained from axenic E. coli culture contained roughly 1.15 nM thiamine pyrophosphate and 4.0-9.1 nM of the thiamine precursor and degradation product, 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP). These compounds were found to promote the growth, lipid content, and glucose uptake of A. protothecoides, while dramatically improving substrate utilization efficiency. Due to widespread cofactor auxotrophy among algae, the co-culture results presented here likely extend to a large number of microbial community systems. We show that algal-algal symbiosis based on cofactor exchange is also possible, opening a new frontier in algae cultivation management. These findings highlight the potential of engineered microbial communities for improved algal biofuel production and wastewater treatment.

AB - Algae have gained attention for production of fuels and chemicals, and treatment of wastewater. The high cost of algae cultivation, however, has limited industry adoption for these applications. Developing methods to increase algal growth rates and lipid content has emerged as an important strategy toward reducing production costs, and significant research effort has been exerted in this area. We have reported previously that co-culturing the green alga, Auxenochlorella protothecoides, with Escherichia coli under mixotrophic conditions led to 2-6 fold increases in algal growth, doubling of neutral lipid content, and elevated nutrient removal rates compared to axenic growth, indicative of a symbiotic relationship. In the present work, we reveal that symbiosis stems largely from E. coli's provision of thiamine derivatives and degradation products to A. protothecoides. LCMS showed that residual cell-free medium obtained from axenic E. coli culture contained roughly 1.15 nM thiamine pyrophosphate and 4.0-9.1 nM of the thiamine precursor and degradation product, 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP). These compounds were found to promote the growth, lipid content, and glucose uptake of A. protothecoides, while dramatically improving substrate utilization efficiency. Due to widespread cofactor auxotrophy among algae, the co-culture results presented here likely extend to a large number of microbial community systems. We show that algal-algal symbiosis based on cofactor exchange is also possible, opening a new frontier in algae cultivation management. These findings highlight the potential of engineered microbial communities for improved algal biofuel production and wastewater treatment.

KW - Biofuel

KW - Co-culture

KW - Cofactor

KW - Organism interaction

KW - Symbiosis

KW - Wastewater treatment

UR - http://www.scopus.com/inward/record.url?scp=84974588379&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84974588379&partnerID=8YFLogxK

U2 - 10.1016/j.algal.2016.05.024

DO - 10.1016/j.algal.2016.05.024

M3 - Article

VL - 17

SP - 308

EP - 315

JO - Algal Research

JF - Algal Research

SN - 2211-9264

ER -