Cocoa procyanidins and human cytokine transcription and secretion

Tin Mao, Judith A Van de Water, Carl L Keen, Harold H. Schmitz, M. Eric Gershwin

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

We examined whether cocoa, in its isolated procyanidin fractions monomer through decamer), would modulate cytokine production at the levels of transcription and protein secretion in both resting and phytohemagglutinin (PHA)-stimulated peripheral blood mononuclear cells (PBMC). In resting cells, interleukin (IL)-1β and IL-4 gene expression from cocoa-treated cells varied markedly among the subjects tested. However, at the protein level, the larger fractions (pentamer through decamer) stimulated a dramatic increase in IL-1β concentration (up to ninefold) with increasing degree of polymerization. Similarly, these larger fractions augmented IL-4 concentration by as much as 2 pg/ml, whereas the control displayed levels nearly undetectable. In the presence of PHA, gene expression also seemed to be most affected by the larger procyanidin fractions. The pentameric through decameric fractions increased IL-1β expression by 7-19% compared with PHA control, whereas the hexameric through decameric fractions significantly inhibited PHA-induced IL-4 transcription in the range of 71-86%. This observation at the transcription level for IL-1β was reflected at the protein level in PHA-stimulated PBMC. Significant reductions in mitogen-induced IL-4 production were also seen at the protein level with the hexamer, heptamer and octamer. Individual oligomeric cocoa fractions were unstimulatory for IL-2 in resting PBMC. However, when induced with PHA, the pentamer, hexamer and heptamer fractions caused a 61-73% inhibition in IL-2 gene expression. This study offers additional data for the consideration of the health benefits of dietary polyphenols from a wide variety of foods, including those benefits associated specifically with cocoa and chocolate consumption.

Original languageEnglish (US)
JournalJournal of Nutrition
Volume130
Issue number8 SUPPL.
StatePublished - 2000

Fingerprint

Proanthocyanidins
procyanidins
phytohemagglutinin
Phytohemagglutinins
cytokines
transcription (genetics)
interleukin-1
secretion
interleukin-4
Cytokines
Interleukin-1
Interleukin-4
mononuclear leukocytes
Blood Cells
interleukin-2
Gene Expression
gene expression
Interleukin-2
Proteins
protein secretion

Keywords

  • Cocoa
  • Cytokines
  • Procyanidin

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Cocoa procyanidins and human cytokine transcription and secretion. / Mao, Tin; Van de Water, Judith A; Keen, Carl L; Schmitz, Harold H.; Gershwin, M. Eric.

In: Journal of Nutrition, Vol. 130, No. 8 SUPPL., 2000.

Research output: Contribution to journalArticle

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