Cocoa inhibits platelet activation and function

Dietrich Rein, Teresa G. Paglieroni, Ted Wun, Debra A. Pearson, Harold H. Schmitz, Robert Gosselin, Carl L. Keen

Research output: Contribution to journalArticle

290 Citations (Scopus)

Abstract

Background: Epidemiologic studies have shown inverse associations between dietary polyphenols and mortality from coronary heart disease. However, the basis for this protective association is uncertain. Food polyphenols reportedly have antioxidant properties and decrease platelet function in vivo. Objective: This study sought to evaluate whether consumption of a polyphenol-rich cocoa beverage modulates human platelet activation and primary hemostasis. Design: Peripheral blood was obtained from 30 healthy subjects before and 2 and 6 h after ingestion of a cocoa beverage (n = 10), a caffeine-containing control beverage (n = 10), or water (n = 10). Platelet activation was measured in terms of expression of activation- dependent platelet antigens and platelet microparticle formation by using fluorescent-labeled monoclonal antibodies and flow cytometry. Primary platelet-related hemostasis was measured with a platelet function analyzer. Results: Ex vivo epinephrine- or ADP-stimulated expression of the fibrinogen- binding conformation of glycoprotein IIb-IIIa was lower 2 and 6 h after consumption of cocoa than before consumption. Cocoa consumption also decreased ADP-stimulated P-selectin expression. In contrast, epinephrine induced platelet glycoprotein IIb-IIIa expression increased after consumption of the caffeine-containing beverage but not after water consumption. Platelet microparticle formation decreased 2 and 6 h after cocoa consumption but increased after caffeine and water consumption. Primary hemostasis in response to epinephrine in vitro was inhibited 6 h after cocoa consumption. The caffeine-containing beverage inhibited ADP-induced primary hemostasis 2 and 6 h after consumption. Conclusions: Cocoa consumption suppressed ADP- or epinephrine-stimulated platelet activation and platelet microparticle formation. Cocoa consumption had an aspirin-like effect on primary hemostasis.

Original languageEnglish (US)
Pages (from-to)30-35
Number of pages6
JournalAmerican Journal of Clinical Nutrition
Volume72
Issue number1
StatePublished - Jul 2000

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platelet activation
hemostasis
Platelet Activation
Beverages
epinephrine
Hemostasis
caffeine
Blood Platelets
Caffeine
Adenosine Diphosphate
Epinephrine
cocoa (beverage)
beverages
Polyphenols
polyphenols
Platelet Glycoprotein GPIIb-IIIa Complex
glycoproteins
Drinking
aspirin
water

Keywords

  • Blood coagulation
  • Cardiovascular disease
  • CD62P
  • Chocolate
  • Cocoa beverage
  • Dietary polyphenols
  • Glycoprotein IIb-IIIa complex
  • Hemostasis
  • P-selectin
  • Platelet activation
  • Platelet function tests
  • Platelet glycoprotein
  • Whole-blood flow cytometry

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Rein, D., Paglieroni, T. G., Wun, T., Pearson, D. A., Schmitz, H. H., Gosselin, R., & Keen, C. L. (2000). Cocoa inhibits platelet activation and function. American Journal of Clinical Nutrition, 72(1), 30-35.

Cocoa inhibits platelet activation and function. / Rein, Dietrich; Paglieroni, Teresa G.; Wun, Ted; Pearson, Debra A.; Schmitz, Harold H.; Gosselin, Robert; Keen, Carl L.

In: American Journal of Clinical Nutrition, Vol. 72, No. 1, 07.2000, p. 30-35.

Research output: Contribution to journalArticle

Rein, D, Paglieroni, TG, Wun, T, Pearson, DA, Schmitz, HH, Gosselin, R & Keen, CL 2000, 'Cocoa inhibits platelet activation and function', American Journal of Clinical Nutrition, vol. 72, no. 1, pp. 30-35.
Rein D, Paglieroni TG, Wun T, Pearson DA, Schmitz HH, Gosselin R et al. Cocoa inhibits platelet activation and function. American Journal of Clinical Nutrition. 2000 Jul;72(1):30-35.
Rein, Dietrich ; Paglieroni, Teresa G. ; Wun, Ted ; Pearson, Debra A. ; Schmitz, Harold H. ; Gosselin, Robert ; Keen, Carl L. / Cocoa inhibits platelet activation and function. In: American Journal of Clinical Nutrition. 2000 ; Vol. 72, No. 1. pp. 30-35.
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AU - Rein, Dietrich

AU - Paglieroni, Teresa G.

AU - Wun, Ted

AU - Pearson, Debra A.

AU - Schmitz, Harold H.

AU - Gosselin, Robert

AU - Keen, Carl L.

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N2 - Background: Epidemiologic studies have shown inverse associations between dietary polyphenols and mortality from coronary heart disease. However, the basis for this protective association is uncertain. Food polyphenols reportedly have antioxidant properties and decrease platelet function in vivo. Objective: This study sought to evaluate whether consumption of a polyphenol-rich cocoa beverage modulates human platelet activation and primary hemostasis. Design: Peripheral blood was obtained from 30 healthy subjects before and 2 and 6 h after ingestion of a cocoa beverage (n = 10), a caffeine-containing control beverage (n = 10), or water (n = 10). Platelet activation was measured in terms of expression of activation- dependent platelet antigens and platelet microparticle formation by using fluorescent-labeled monoclonal antibodies and flow cytometry. Primary platelet-related hemostasis was measured with a platelet function analyzer. Results: Ex vivo epinephrine- or ADP-stimulated expression of the fibrinogen- binding conformation of glycoprotein IIb-IIIa was lower 2 and 6 h after consumption of cocoa than before consumption. Cocoa consumption also decreased ADP-stimulated P-selectin expression. In contrast, epinephrine induced platelet glycoprotein IIb-IIIa expression increased after consumption of the caffeine-containing beverage but not after water consumption. Platelet microparticle formation decreased 2 and 6 h after cocoa consumption but increased after caffeine and water consumption. Primary hemostasis in response to epinephrine in vitro was inhibited 6 h after cocoa consumption. The caffeine-containing beverage inhibited ADP-induced primary hemostasis 2 and 6 h after consumption. Conclusions: Cocoa consumption suppressed ADP- or epinephrine-stimulated platelet activation and platelet microparticle formation. Cocoa consumption had an aspirin-like effect on primary hemostasis.

AB - Background: Epidemiologic studies have shown inverse associations between dietary polyphenols and mortality from coronary heart disease. However, the basis for this protective association is uncertain. Food polyphenols reportedly have antioxidant properties and decrease platelet function in vivo. Objective: This study sought to evaluate whether consumption of a polyphenol-rich cocoa beverage modulates human platelet activation and primary hemostasis. Design: Peripheral blood was obtained from 30 healthy subjects before and 2 and 6 h after ingestion of a cocoa beverage (n = 10), a caffeine-containing control beverage (n = 10), or water (n = 10). Platelet activation was measured in terms of expression of activation- dependent platelet antigens and platelet microparticle formation by using fluorescent-labeled monoclonal antibodies and flow cytometry. Primary platelet-related hemostasis was measured with a platelet function analyzer. Results: Ex vivo epinephrine- or ADP-stimulated expression of the fibrinogen- binding conformation of glycoprotein IIb-IIIa was lower 2 and 6 h after consumption of cocoa than before consumption. Cocoa consumption also decreased ADP-stimulated P-selectin expression. In contrast, epinephrine induced platelet glycoprotein IIb-IIIa expression increased after consumption of the caffeine-containing beverage but not after water consumption. Platelet microparticle formation decreased 2 and 6 h after cocoa consumption but increased after caffeine and water consumption. Primary hemostasis in response to epinephrine in vitro was inhibited 6 h after cocoa consumption. The caffeine-containing beverage inhibited ADP-induced primary hemostasis 2 and 6 h after consumption. Conclusions: Cocoa consumption suppressed ADP- or epinephrine-stimulated platelet activation and platelet microparticle formation. Cocoa consumption had an aspirin-like effect on primary hemostasis.

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KW - Platelet glycoprotein

KW - Whole-blood flow cytometry

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