Cocoa flavanols lower vascular arginase activity in human endothelial cells in vitro and in erythrocytes in vivo

Oliver Schnorr, Tatjana Brossette, Tony Y. Momma, Petra Kleinbongard, Carl L Keen, Hagen Schroeter, Helmut Sies

Research output: Contribution to journalArticle

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Abstract

The availability of l-arginine can be a rate-limiting factor for cellular NO production by nitric oxide synthases (NOS). Arginase competes with NOS for l-arginine as the common substrate. Increased arginase activity has been linked to low NO levels, and an inhibition of arginase activity has been reported to improve endothelium-dependent vasorelaxation. Based on the above, we hypothesized that an increase in the circulating NO pool following flavanol consumption could be correlated with decreased arginase activity. To test this hypothesis we (a) investigated the effects of (-)-epicatechin and its structurally related metabolites on endothelial arginase expression and activity in vitro; (b) evaluated the effects of dietary flavanol-rich cocoa on kidney arginase activity in vivo; and (c) assessed human erythrocyte arginase activity following flavanol-rich cocoa beverage consumption in a double-blind intervention study with cross-over design. The results demonstrate that cocoa flavanols lower arginase-2 mRNA expression and activity in HUVEC. Dietary intervention with flavanol-rich cocoa caused diminished arginase activity in rat kidney and, erythrocyte arginase activity was lowered in healthy humans following consumption of a high flavanol beverage in vivo.

Original languageEnglish (US)
Pages (from-to)211-215
Number of pages5
JournalArchives of Biochemistry and Biophysics
Volume476
Issue number2
DOIs
StatePublished - Aug 15 2008

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Cocoa
Arginase
Endothelial cells
Human Activities
Blood Vessels
Endothelial Cells
Erythrocytes
Beverages
Nitric Oxide Synthase
Arginine
In Vitro Techniques
Kidney
Catechin
Metabolites
Double-Blind Method
Vasodilation
Cross-Over Studies
Endothelium
Rats

Keywords

  • (-)-Epicatechin
  • Arginase
  • Cocoa
  • Endothelium
  • Erythrocytes
  • Flavanols
  • l-Arginine
  • Nitric oxide

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Schnorr, O., Brossette, T., Momma, T. Y., Kleinbongard, P., Keen, C. L., Schroeter, H., & Sies, H. (2008). Cocoa flavanols lower vascular arginase activity in human endothelial cells in vitro and in erythrocytes in vivo. Archives of Biochemistry and Biophysics, 476(2), 211-215. https://doi.org/10.1016/j.abb.2008.02.040

Cocoa flavanols lower vascular arginase activity in human endothelial cells in vitro and in erythrocytes in vivo. / Schnorr, Oliver; Brossette, Tatjana; Momma, Tony Y.; Kleinbongard, Petra; Keen, Carl L; Schroeter, Hagen; Sies, Helmut.

In: Archives of Biochemistry and Biophysics, Vol. 476, No. 2, 15.08.2008, p. 211-215.

Research output: Contribution to journalArticle

Schnorr, O, Brossette, T, Momma, TY, Kleinbongard, P, Keen, CL, Schroeter, H & Sies, H 2008, 'Cocoa flavanols lower vascular arginase activity in human endothelial cells in vitro and in erythrocytes in vivo', Archives of Biochemistry and Biophysics, vol. 476, no. 2, pp. 211-215. https://doi.org/10.1016/j.abb.2008.02.040
Schnorr, Oliver ; Brossette, Tatjana ; Momma, Tony Y. ; Kleinbongard, Petra ; Keen, Carl L ; Schroeter, Hagen ; Sies, Helmut. / Cocoa flavanols lower vascular arginase activity in human endothelial cells in vitro and in erythrocytes in vivo. In: Archives of Biochemistry and Biophysics. 2008 ; Vol. 476, No. 2. pp. 211-215.
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