Cocoa bean cell and embryo culture

Ming Che Wen, Bruce German, John E. Kinsella

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Callus culture of cocoa bean was initiated from immature cotyledons on agar medium. By dispersing these callus cells, a liquid suspension culture was established. The lipid composition of cocoa suspension culture was investigated and compared with those of cocoa beans of different maturities. Factors affecting fatty acid and triglyceride synthesis in cocoa suspension cultures also were studied. In parallel studies, asexual embryos were proliferated from immature zygotic embryos cultured in semisolid or liquid medium. The factors affecting the lipid composition in cultured embryos are discussed.

Original languageEnglish (US)
Pages (from-to)1720-1724
Number of pages5
JournalJournal of the American Oil Chemists' Society
Issue number11
StatePublished - Nov 1984
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Food Science


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