Cocoa bean cell and embryo culture

Ming Che Wen; Bruce German; John E. Kinsella

doi:10.1007/BF02582135

Cocoa bean cell and embryo culture

Ming Che Wen, Bruce German, John E. Kinsella

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Callus culture of cocoa bean was initiated from immature cotyledons on agar medium. By dispersing these callus cells, a liquid suspension culture was established. The lipid composition of cocoa suspension culture was investigated and compared with those of cocoa beans of different maturities. Factors affecting fatty acid and triglyceride synthesis in cocoa suspension cultures also were studied. In parallel studies, asexual embryos were proliferated from immature zygotic embryos cultured in semisolid or liquid medium. The factors affecting the lipid composition in cultured embryos are discussed.

Original language	English (US)
Pages (from-to)	1720-1724
Number of pages	5
Journal	Journal of the American Oil Chemists' Society
Volume	61
Issue number	11
DOIs	https://doi.org/10.1007/BF02582135
State	Published - Nov 1984
Externally published	Yes

ASJC Scopus subject areas

Chemistry (miscellaneous)
Food Science

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abstract = "Callus culture of cocoa bean was initiated from immature cotyledons on agar medium. By dispersing these callus cells, a liquid suspension culture was established. The lipid composition of cocoa suspension culture was investigated and compared with those of cocoa beans of different maturities. Factors affecting fatty acid and triglyceride synthesis in cocoa suspension cultures also were studied. In parallel studies, asexual embryos were proliferated from immature zygotic embryos cultured in semisolid or liquid medium. The factors affecting the lipid composition in cultured embryos are discussed.",

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