Coating of VEGF-releasing scaffolds with bioactive glass for angiogenesis and bone regeneration

Jonathan K Leach, Darnell Kaigler, Zhuo Wang, Paul H. Krebsbach, David J. Mooney

Research output: Contribution to journalArticle

272 Citations (Scopus)

Abstract

Bioactive glasses are potentially useful as bone defect fillers, and vascular endothelial growth factor (VEGF) has demonstrated benefit in bone regeneration as well. We hypothesized that the specific combination of prolonged localized VEGF presentation from a matrix coated with a bioactive glass may enhance bone regeneration. To test this hypothesis, the capacity of VEGF-releasing polymeric scaffolds with a bioactive glass coating was examined in vitro and in vivo using a rat critical-sized defect model. In the presence of a bioactive glass coating, we did not detect pronounced differences in the differentiation of human mesenchymal stem cells in vitro. However, we observed significantly enhanced mitogenic stimulation of endothelial cells in the presence of the bioactive glass coating, with an additive effect with VEGF release. This trend was maintained in vivo, where coated VEGF-releasing scaffolds demonstrated significant improvements in blood vessel density at 2 weeks versus coated control scaffolds. At 12 weeks, bone mineral density was significantly increased in coated VEGF-releasing scaffolds versus coated controls, while only a slight increase in bone volume fraction was observed. The results of this study suggest that a bioactive glass coating on a polymeric substrate participates in bone healing through indirect processes which enhance angiogenesis and bone maturation and not directly on osteoprogenitor differentiation and bone formation. The mass of bioactive glass used in this study provides a comparable and potentially additive, response to localized VEGF delivery over early time points. These studies demonstrate a materials approach to achieve an angiogenic response formerly limited to the delivery of inductive growth factors.

Original languageEnglish (US)
Pages (from-to)3249-3255
Number of pages7
JournalBiomaterials
Volume27
Issue number17
DOIs
StatePublished - Jun 2006
Externally publishedYes

Fingerprint

Bioactive glass
Bone Regeneration
Scaffolds
Vascular Endothelial Growth Factor A
Glass
Bone
Coatings
Bone and Bones
Defects
Intercellular Signaling Peptides and Proteins
Mesenchymal Stromal Cells
Osteogenesis
Endothelial cells
Blood vessels
Bone Density
Blood Vessels
Stem cells
Minerals
Endothelial Cells
Fillers

Keywords

  • Angiogenesis
  • Bioactive glass
  • Bone regeneration
  • VEGF

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Coating of VEGF-releasing scaffolds with bioactive glass for angiogenesis and bone regeneration. / Leach, Jonathan K; Kaigler, Darnell; Wang, Zhuo; Krebsbach, Paul H.; Mooney, David J.

In: Biomaterials, Vol. 27, No. 17, 06.2006, p. 3249-3255.

Research output: Contribution to journalArticle

Leach, Jonathan K ; Kaigler, Darnell ; Wang, Zhuo ; Krebsbach, Paul H. ; Mooney, David J. / Coating of VEGF-releasing scaffolds with bioactive glass for angiogenesis and bone regeneration. In: Biomaterials. 2006 ; Vol. 27, No. 17. pp. 3249-3255.
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