Co-translational protein targeting facilitates centrosomal recruitment of PCNT during centrosome maturation in vertebrates

Guadalupe Sepulveda, Mark Antkowiak, Ingrid Brust-Mascher, Karan Mahe, Tingyoung Ou, Noemi M. Castro, Lana N. Christensen, Lee Cheung, Xueer Jiang, Daniel Yoon, Bo Huang, Li-En Jao

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

As microtubule-organizing centers of animal cells, centrosomes guide the formation of the bipolar spindle that segregates chromosomes during mitosis. At mitosis onset, centrosomes maximize microtubule-organizing activity by rapidly expanding the pericentriolar material (PCM). This process is in part driven by the large PCM protein pericentrin (PCNT), as its level increases at the PCM and helps recruit additional PCM components. However, the mechanism underlying the timely centrosomal enrichment of PCNT remains unclear. Here, we show that PCNT is delivered co-translationally to centrosomes during early mitosis by cytoplasmic dynein, as evidenced by centrosomal enrichment of PCNT mRNA, its translation near centrosomes, and requirement of intact polysomes for PCNT mRNA localization. Additionally, the microtubule minus-end regulator, ASPM, is also targeted co-translationally to mitotic spindle poles. Together, these findings suggest that co-translational targeting of cytoplasmic proteins to specific subcellular destinations may be a generalized protein targeting mechanism.

Original languageEnglish (US)
Article numbere34959
JournaleLife
Volume7
DOIs
StatePublished - Apr 30 2018

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Centrosome
Protein Transport
Vertebrates
Mitosis
Proteins
Microtubules
Cytoplasmic Dyneins
Microtubule-Organizing Center
Spindle Poles
Messenger RNA
Spindle Apparatus
Polyribosomes
Protein Biosynthesis
Chromosomes
Poles
Animals
Cells
pericentrin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Co-translational protein targeting facilitates centrosomal recruitment of PCNT during centrosome maturation in vertebrates. / Sepulveda, Guadalupe; Antkowiak, Mark; Brust-Mascher, Ingrid; Mahe, Karan; Ou, Tingyoung; Castro, Noemi M.; Christensen, Lana N.; Cheung, Lee; Jiang, Xueer; Yoon, Daniel; Huang, Bo; Jao, Li-En.

In: eLife, Vol. 7, e34959, 30.04.2018.

Research output: Contribution to journalArticle

Sepulveda, G, Antkowiak, M, Brust-Mascher, I, Mahe, K, Ou, T, Castro, NM, Christensen, LN, Cheung, L, Jiang, X, Yoon, D, Huang, B & Jao, L-E 2018, 'Co-translational protein targeting facilitates centrosomal recruitment of PCNT during centrosome maturation in vertebrates', eLife, vol. 7, e34959. https://doi.org/10.7554/eLife.34959
Sepulveda, Guadalupe ; Antkowiak, Mark ; Brust-Mascher, Ingrid ; Mahe, Karan ; Ou, Tingyoung ; Castro, Noemi M. ; Christensen, Lana N. ; Cheung, Lee ; Jiang, Xueer ; Yoon, Daniel ; Huang, Bo ; Jao, Li-En. / Co-translational protein targeting facilitates centrosomal recruitment of PCNT during centrosome maturation in vertebrates. In: eLife. 2018 ; Vol. 7.
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