Co-translational protein targeting catalyzed by the Escherichia coli signal recognition particle and its receptor

Ted Powers, Peter Walter

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

The Ffh-4.5S ribonucleoprotein particle (RNP) and FtsY from Escherichia coli are homologous to essential components of the mammalian signal recognition particle (SRP) and SRP receptor, respectively. The ability of these E.coli components to function in a bona fide co-translational targeting pathway remains unclear. Here we demonstrate that the Ffh-4.5S RNP and FtsY can efficiently replace their mammalian counterparts in targeting nascent secretory proteins to microsomal membranes in vitro. Targeting in the heterologous system requires a hydrophobic signal sequence, utilizes GTP and, moreover, occurs co-translationally. Unlike mammalian SRP, however, the Ffh-4.5S RNP is unable to arrest translational elongation, which results in a narrow time window for the ribosome nascent chain to interact productively with the membrane-bound translocation machinery. The highly negatively charged N-terminal domain of FtsY, which is a conserved feature among prokaryotic SRP receptor homologs, is important for translocation and acts to localize the protein to the membrane. Our data illustrate the extreme functional conservation between prokaryotic and eukaryotic SRP and SRP receptors and suggest that the basic mechanism of co-translational protein targeting is conserved between bacteria and mammals.

Original languageEnglish (US)
Pages (from-to)4880-4886
Number of pages7
JournalEMBO Journal
Volume16
Issue number16
DOIs
StatePublished - Aug 15 1997

Fingerprint

Signal Recognition Particle
Ribonucleoproteins
Protein Transport
Escherichia coli
Membranes
Proteins
Mammals
Protein Sorting Signals
Guanosine Triphosphate
Ribosomes
Machinery
Elongation
Conservation
Bacteria
Membrane Proteins
signal peptide receptor

Keywords

  • Co-translational
  • Functional conservation
  • Protein targeting
  • Signal recognition particle
  • SRP receptor
  • Translocation

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Co-translational protein targeting catalyzed by the Escherichia coli signal recognition particle and its receptor. / Powers, Ted; Walter, Peter.

In: EMBO Journal, Vol. 16, No. 16, 15.08.1997, p. 4880-4886.

Research output: Contribution to journalArticle

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