Closed circular viral DNA and asymmetrical heterogeneous forms in livers from animals infected with ground squirrel hepatitis virus

Barbara Weiser, D. Ganem, C. Seeger, H. E. Varmus

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

To identify possible intermediates in the replication of ground squirrel hepatitis virus, we characterized the major forms of intracellular virus-specific DNA in the livers of infected ground squirrels. A variety of DNA species was found: covalently closed circular molecules, relaxed circular molecules, and a heterogeneous collection of molecules that migrated ahead of closed circular DNA during agarose gel electrophoresis. The heterogeneous DNA was at least partly single stranded, consisted of minus strands in a greater than 8-fold mass excess of plus strands, and was tightly associated with protein.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalJournal of Virology
Volume48
Issue number1
StatePublished - 1983
Externally publishedYes

Fingerprint

Ground squirrel hepatitis virus
Circular DNA
Sciuridae
Hepatitis Viruses
Viral DNA
liver
DNA Viruses
Agar Gel Electrophoresis
Liver
DNA
circular DNA
animals
squirrels
agarose
gel electrophoresis
viruses
Proteins
proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Closed circular viral DNA and asymmetrical heterogeneous forms in livers from animals infected with ground squirrel hepatitis virus. / Weiser, Barbara; Ganem, D.; Seeger, C.; Varmus, H. E.

In: Journal of Virology, Vol. 48, No. 1, 1983, p. 1-9.

Research output: Contribution to journalArticle

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