To identify possible intermediates in the replication of ground squirrel hepatitis virus, we characterized the major forms of intracellular virus-specific DNA in the livers of infected ground squirrels. A variety of DNA species was found: covalently closed circular molecules, relaxed circular molecules, and a heterogeneous collection of molecules that migrated ahead of closed circular DNA during agarose gel electrophoresis. The heterogeneous DNA was at least partly single stranded, consisted of minus strands in a greater than 8-fold mass excess of plus strands, and was tightly associated with protein.
|Original language||English (US)|
|Number of pages||9|
|Journal||Journal of Virology|
|State||Published - 1983|
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