Closed circular viral DNA and asymmetrical heterogeneous forms in livers from animals infected with ground squirrel hepatitis virus

Barbara Weiser, D. Ganem, C. Seeger, H. E. Varmus

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

To identify possible intermediates in the replication of ground squirrel hepatitis virus, we characterized the major forms of intracellular virus-specific DNA in the livers of infected ground squirrels. A variety of DNA species was found: covalently closed circular molecules, relaxed circular molecules, and a heterogeneous collection of molecules that migrated ahead of closed circular DNA during agarose gel electrophoresis. The heterogeneous DNA was at least partly single stranded, consisted of minus strands in a greater than 8-fold mass excess of plus strands, and was tightly associated with protein.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalJournal of Virology
Volume48
Issue number1
StatePublished - 1983
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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