Cloning, structural analysis and mapping of the mouse selenocysteine tRNA[Ser]Sec gene (Trsp)

Michael R. Bösl, Michael F Seldin, Susumu Nishimura, Michael F. Taketo

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

The tRNA[Ser]Sec molecule mediates the synthesis of selenoproteins by incorporating selenocysteine into specific UGA codons upon translation of mRNAs that encode selenocysteine-containing proteins. The mouse gene encoding tRNA[Ser]Sec (Trsp) was isolated from a genomic library and sequenced. The mouse sequence is colinear with its tRNA product, and contains a C to T transition relative to the homologous genes in other vertebrates except rat. Transcriptional control motifs found 5′ to the tRNA coding region included a TATA element, a PSE element and an SPH motif which is associated with an octamer motif. A Northern hybridization analysis showed highest expression in the testis, followed by thymus, spleen, kidney, ovary, brain, liver, heart and skeletal muscle. Surprisingly, the expression level was lowest in embryonic stem cells. These results suggest a tissue-specific transcriptional control. Using restriction fragment length variants (RFLVs) in interspecific backcross mice between Mus musculus (C3H strain) and Mus spretus, the Trsp gene was mapped to the proximal region of mouse Chr 7, cosegregating with octamer-binding transcription factor-2 (Otf2).

Original languageEnglish (US)
Pages (from-to)247-252
Number of pages6
JournalMGG Molecular & General Genetics
Volume248
Issue number3
DOIs
StatePublished - Aug 1995
Externally publishedYes

Keywords

  • Glutathione peroxidase
  • Linkage mapping
  • RFLV
  • Selenocysteine tRNA
  • Selenoprotein

ASJC Scopus subject areas

  • Genetics

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