Cloning, sequencing and function of sanA, a gene involved in nikkomycin biosynthesis of Streptomyces ansochromogenes

Junyong Jia, Wenli Li, Wei Chen, Liping Nie, Huarong Tan

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Abstract

Several genetically stable mutants blocked in nikkomycin biosynthesis were obtained after the slightly germinated spores of Streptomyces ansochromogenes, a nikkomycin producer, were treated with ultra violet radiation. One of the mutants is the same in morpholotical differentiation as the wild type strain and is designated as NBB19. A DNA library was constructed using plasmid plJ702 as cloning vector, NBB19 as cloning recipient. A 6 kb DNA fragment which can genetically complement NBB19 was cloned when screening the library for antifungal activity. Sequence analysis showed that the 3 kb Bgl II-Sal I fragment contains one complete ORF (ORF1) and one partial ORF (ORF2). ORF1 is designated as sanA. sanA is 1 365 bp, encoding a protein consisting of 454 amino acid residues. Database searching indicated that sanA is homologous to the hypothetical methyltransferase in Pyrococcus horikoshii with 25% identities and 41% positives. Disruptant of sanA lost the ability to synthesize nikkomycin. It indicated that sanA is a novel gene which is essential for nikkomycin biosynthesis.

Original languageEnglish (US)
Pages (from-to)37-38
Number of pages2
JournalScience in China, Series C: Life Sciences
Volume43
Issue number1
StatePublished - 2000
Externally publishedYes

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Keywords

  • Complementary cloning
  • Nikkomycin biosynthesis gene
  • Streptomyces

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

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