Abstract
Several genetically stable mutants blocked in nikkomycin biosynthesis were obtained after the slightly germinated spores of Streptomyces ansochromogenes, a nikkomycin producer, were treated with ultra violet radiation. One of the mutants is the same in morpholotical differentiation as the wild type strain and is designated as NBB19. A DNA library was constructed using plasmid plJ702 as cloning vector, NBB19 as cloning recipient. A 6 kb DNA fragment which can genetically complement NBB19 was cloned when screening the library for antifungal activity. Sequence analysis showed that the 3 kb Bgl II-Sal I fragment contains one complete ORF (ORF1) and one partial ORF (ORF2). ORF1 is designated as sanA. sanA is 1 365 bp, encoding a protein consisting of 454 amino acid residues. Database searching indicated that sanA is homologous to the hypothetical methyltransferase in Pyrococcus horikoshii with 25% identities and 41% positives. Disruptant of sanA lost the ability to synthesize nikkomycin. It indicated that sanA is a novel gene which is essential for nikkomycin biosynthesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 37-38 |
| Number of pages | 2 |
| Journal | Science in China, Series C: Life Sciences |
| Volume | 43 |
| Issue number | 1 |
| State | Published - 2000 |
| Externally published | Yes |
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Keywords
- Complementary cloning
- Nikkomycin biosynthesis gene
- Streptomyces
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agricultural and Biological Sciences (miscellaneous)
Cite this
Cloning, sequencing and function of sanA, a gene involved in nikkomycin biosynthesis of Streptomyces ansochromogenes. / Jia, Junyong; Li, Wenli; Chen, Wei; Nie, Liping; Tan, Huarong.
In: Science in China, Series C: Life Sciences, Vol. 43, No. 1, 2000, p. 37-38.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cloning, sequencing and function of sanA, a gene involved in nikkomycin biosynthesis of Streptomyces ansochromogenes
AU - Jia, Junyong
AU - Li, Wenli
AU - Chen, Wei
AU - Nie, Liping
AU - Tan, Huarong
PY - 2000
Y1 - 2000
N2 - Several genetically stable mutants blocked in nikkomycin biosynthesis were obtained after the slightly germinated spores of Streptomyces ansochromogenes, a nikkomycin producer, were treated with ultra violet radiation. One of the mutants is the same in morpholotical differentiation as the wild type strain and is designated as NBB19. A DNA library was constructed using plasmid plJ702 as cloning vector, NBB19 as cloning recipient. A 6 kb DNA fragment which can genetically complement NBB19 was cloned when screening the library for antifungal activity. Sequence analysis showed that the 3 kb Bgl II-Sal I fragment contains one complete ORF (ORF1) and one partial ORF (ORF2). ORF1 is designated as sanA. sanA is 1 365 bp, encoding a protein consisting of 454 amino acid residues. Database searching indicated that sanA is homologous to the hypothetical methyltransferase in Pyrococcus horikoshii with 25% identities and 41% positives. Disruptant of sanA lost the ability to synthesize nikkomycin. It indicated that sanA is a novel gene which is essential for nikkomycin biosynthesis.
AB - Several genetically stable mutants blocked in nikkomycin biosynthesis were obtained after the slightly germinated spores of Streptomyces ansochromogenes, a nikkomycin producer, were treated with ultra violet radiation. One of the mutants is the same in morpholotical differentiation as the wild type strain and is designated as NBB19. A DNA library was constructed using plasmid plJ702 as cloning vector, NBB19 as cloning recipient. A 6 kb DNA fragment which can genetically complement NBB19 was cloned when screening the library for antifungal activity. Sequence analysis showed that the 3 kb Bgl II-Sal I fragment contains one complete ORF (ORF1) and one partial ORF (ORF2). ORF1 is designated as sanA. sanA is 1 365 bp, encoding a protein consisting of 454 amino acid residues. Database searching indicated that sanA is homologous to the hypothetical methyltransferase in Pyrococcus horikoshii with 25% identities and 41% positives. Disruptant of sanA lost the ability to synthesize nikkomycin. It indicated that sanA is a novel gene which is essential for nikkomycin biosynthesis.
KW - Complementary cloning
KW - Nikkomycin biosynthesis gene
KW - Streptomyces
UR - http://www.scopus.com/inward/record.url?scp=23044461940&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=23044461940&partnerID=8YFLogxK
M3 - Article
VL - 43
SP - 37
EP - 38
JO - Science in China, Series C: Life Sciences
JF - Science in China, Series C: Life Sciences
SN - 1006-9305
IS - 1
ER -