Cloning, sequencing and function of sanA, a gene involved in nikkomycin biosynthesis of Streptomyces ansochromogenes

Junyong Jia, Wenli Li, Wei Chen, Liping Nie, Huarong Tan

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Several genetically stable mutants blocked in nikkomycin biosynthesis were obtained after the slightly germinated spores of Streptomyces ansochromogenes, a nikkomycin producer, were treated with ultra violet radiation. One of the mutants is the same in morpholotical differentiation as the wild type strain and is designated as NBB19. A DNA library was constructed using plasmid plJ702 as cloning vector, NBB19 as cloning recipient. A 6 kb DNA fragment which can genetically complement NBB19 was cloned when screening the library for antifungal activity. Sequence analysis showed that the 3 kb Bgl II-Sal I fragment contains one complete ORF (ORF1) and one partial ORF (ORF2). ORF1 is designated as sanA. sanA is 1 365 bp, encoding a protein consisting of 454 amino acid residues. Database searching indicated that sanA is homologous to the hypothetical methyltransferase in Pyrococcus horikoshii with 25% identities and 41% positives. Disruptant of sanA lost the ability to synthesize nikkomycin. It indicated that sanA is a novel gene which is essential for nikkomycin biosynthesis.

Original languageEnglish (US)
Pages (from-to)37-38
Number of pages2
JournalScience in China, Series C: Life Sciences
Volume43
Issue number1
StatePublished - 2000
Externally publishedYes

Fingerprint

Streptomyces ansochromogenes
nikkomycins
Cloning
Biosynthesis
Streptomyces
Organism Cloning
molecular cloning
Genes
biosynthesis
DNA
gene
ultraviolet radiation
plasmid
Open Reading Frames
open reading frames
Pyrococcus horikoshii
spore
genes
amino acid
genetic vectors

Keywords

  • Complementary cloning
  • Nikkomycin biosynthesis gene
  • Streptomyces

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Cloning, sequencing and function of sanA, a gene involved in nikkomycin biosynthesis of Streptomyces ansochromogenes. / Jia, Junyong; Li, Wenli; Chen, Wei; Nie, Liping; Tan, Huarong.

In: Science in China, Series C: Life Sciences, Vol. 43, No. 1, 2000, p. 37-38.

Research output: Contribution to journalArticle

Jia, Junyong ; Li, Wenli ; Chen, Wei ; Nie, Liping ; Tan, Huarong. / Cloning, sequencing and function of sanA, a gene involved in nikkomycin biosynthesis of Streptomyces ansochromogenes. In: Science in China, Series C: Life Sciences. 2000 ; Vol. 43, No. 1. pp. 37-38.
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AU - Tan, Huarong

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