TY - JOUR
T1 - Cloning of a Parathyroid Hormone/Parathyroid Hormone-Related Peptide Receptor (PTHR) cDNA from a Rat Osteosarcoma (UMR 106) Cell Line
T2 - Chromosomal Assignment of the Gene in the Human, Mouse, and Rat Genomes
AU - Pausova, Zdenka
AU - Bourdon, Johanne
AU - Clayton, Dale
AU - Mattel, Marie Geneviève
AU - Seldin, Michael F
AU - Janicic, Natasa
AU - Rivière, Michèle
AU - Szpirer, Josiane
AU - Levan, Goran
AU - Szpirer, Claude
AU - Goltzman, David
AU - Hendy, Geoffrey N.
PY - 1994/3/1
Y1 - 1994/3/1
N2 - Complementary DNAs spanning the entire coding region of the rat parathyroid hormone/parathyroid hormone-related peptide receptor (PTHR) were isolated from a rat osteosarcoma (UMR 106) cell-line cDNA library. The longest of these clones (rPTHrec4) was used to chromosomally assign the PTHR gene in the human, rat, and mouse genomes. By somatic cell hybrid analysis, the gene was localized to human chromosome 3 and rat chromosome 8; by in situ hybridization, the gene was mapped to human chromosome 3p21.1-p22 and to mouse chromosome 9 band F; and by interspecific backcross analysis, the Pthr gene segregated with the transferrin (Trf) gene in chromosome 9 band F. Mouse chromosome 9 and rat chromosome 8 are known to be highly homologous and to also show synteny conservation with human chromosome 3. These three chromosomes share the transferrin gene (TF), the myosin light polypeptide 3 gene (MYL3), and the acylpeptide hydrolase gene (APEH). Our results add a fourth gene, the PTHR gene, to the synteny group conserved in these chromosomes.
AB - Complementary DNAs spanning the entire coding region of the rat parathyroid hormone/parathyroid hormone-related peptide receptor (PTHR) were isolated from a rat osteosarcoma (UMR 106) cell-line cDNA library. The longest of these clones (rPTHrec4) was used to chromosomally assign the PTHR gene in the human, rat, and mouse genomes. By somatic cell hybrid analysis, the gene was localized to human chromosome 3 and rat chromosome 8; by in situ hybridization, the gene was mapped to human chromosome 3p21.1-p22 and to mouse chromosome 9 band F; and by interspecific backcross analysis, the Pthr gene segregated with the transferrin (Trf) gene in chromosome 9 band F. Mouse chromosome 9 and rat chromosome 8 are known to be highly homologous and to also show synteny conservation with human chromosome 3. These three chromosomes share the transferrin gene (TF), the myosin light polypeptide 3 gene (MYL3), and the acylpeptide hydrolase gene (APEH). Our results add a fourth gene, the PTHR gene, to the synteny group conserved in these chromosomes.
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U2 - 10.1006/geno.1994.1122
DO - 10.1006/geno.1994.1122
M3 - Article
C2 - 8020952
AN - SCOPUS:0028288301
VL - 20
SP - 20
EP - 26
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 1
ER -