TY - JOUR
T1 - Cloning of a Novel Putative Protein Kinase Having a Leucine Zipper Domain from Human Brain
AU - Reddy, U. R.
AU - Pleasure, David E
PY - 1994/7/15
Y1 - 1994/7/15
N2 - We report a novel putative serine/threonine protein kinase containing a leucine-zipper domain, isolated from an human neuronal cell line. The teratocarcinoma cell line NT2 was differentiated to postmitotic NT2-N neurons by treatment with retinoic acid, and degenerate oligonucleotide primers to the catalytic domains of protein kinases were employed to PCR amplify subtractive cDNAs. We identified a clone, represented at higher abundance in NT2-N neurons than in the parental cell line, which encodes a putative serine/threonine kinase of 668 aminoacids, the leucine-zipper protein kinase (zpk). Zpk protein contains a leucine-zipper domain, found in many DNA-binding proteins, but few protein kinases. Steady-state mRNA levels for zpk are high in human brain and kidney. Further studies are required to evaluate the role of zpk in neuronal differentiation.
AB - We report a novel putative serine/threonine protein kinase containing a leucine-zipper domain, isolated from an human neuronal cell line. The teratocarcinoma cell line NT2 was differentiated to postmitotic NT2-N neurons by treatment with retinoic acid, and degenerate oligonucleotide primers to the catalytic domains of protein kinases were employed to PCR amplify subtractive cDNAs. We identified a clone, represented at higher abundance in NT2-N neurons than in the parental cell line, which encodes a putative serine/threonine kinase of 668 aminoacids, the leucine-zipper protein kinase (zpk). Zpk protein contains a leucine-zipper domain, found in many DNA-binding proteins, but few protein kinases. Steady-state mRNA levels for zpk are high in human brain and kidney. Further studies are required to evaluate the role of zpk in neuronal differentiation.
UR - http://www.scopus.com/inward/record.url?scp=0028037972&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028037972&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1994.1972
DO - 10.1006/bbrc.1994.1972
M3 - Article
C2 - 8037767
AN - SCOPUS:0028037972
VL - 202
SP - 613
EP - 620
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -