Cloning of a novel C-type lectin expressed by murine macrophages

Signe G. Balch, Andrew J. McKnight, Michael F Seldin, Siamon Gordon

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

We report the cloning of a novel macrophage-restricted C-type lectin by differential display polymerase chain reaction. This molecule, named mouse macrophage C-type lectin, is a 219-amino acid, type II transmembrane protein with a single extracellular C-type lectin domain. Northern blot analysis indicates that it is expressed in cell lines and normal mouse tissues in a macrophage-restricted manner. The cDNA and genomic sequences of mouse macrophage C-type lectin indicate that it is related to the Group II animal C-type lectins. The mcl gene locus has been mapped between the genes for the interleukin-17 receptor and CD4 on mouse chromosome 6, the same chromosome as the mouse natural killer cell gene complex.

Original languageEnglish (US)
Pages (from-to)18656-18664
Number of pages9
JournalJournal of Biological Chemistry
Volume273
Issue number29
DOIs
StatePublished - Jul 17 1998

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C-Type Lectins
Macrophages
Cloning
Organism Cloning
Genes
Chromosomes
Interleukin-17 Receptors
Chromosomes, Human, Pair 6
Polymerase chain reaction
Lectins
Natural Killer Cells
Northern Blotting
Animals
Complementary DNA
Display devices
Cells
Tissue
Amino Acids
Cell Line
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Biochemistry

Cite this

Cloning of a novel C-type lectin expressed by murine macrophages. / Balch, Signe G.; McKnight, Andrew J.; Seldin, Michael F; Gordon, Siamon.

In: Journal of Biological Chemistry, Vol. 273, No. 29, 17.07.1998, p. 18656-18664.

Research output: Contribution to journalArticle

Balch, Signe G. ; McKnight, Andrew J. ; Seldin, Michael F ; Gordon, Siamon. / Cloning of a novel C-type lectin expressed by murine macrophages. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 29. pp. 18656-18664.
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