Cloning and sequencing of human k-casein cDNA

Sven Bergström, Lennart Hansson, Olle Hernell, Bo Lönnerdal, Anna Karin Nilsson, Mats Strömqvist

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

A cDNA encoding K-casein of human milk was cloned and sequenced. The K-casein cDNA was isolated from a λgt11 library generated from mRNA prepared from a mammary gland biopsy obtained from a lactating woman. The library was screened with polyclonal rabbit antibodies raised against purified native K-casein. The obtained nucleotide sequence contained an ORF sufficient to encode the entire amino acid sequence of a K-casein precursor protein consisting of 182 amino acids. This includes a tentative signal peptide of 20 amino acids and a processed protein of 162 amino acids.

Original languageEnglish (US)
Pages (from-to)245-246
Number of pages2
JournalMitochondrial DNA
Volume3
Issue number4
DOIs
StatePublished - 1992

Keywords

  • CDNA cloning
  • Human milk protein
  • K-casein
  • K-protein
  • Nucleotide sequence

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biochemistry
  • Endocrinology

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  • Cite this

    Bergström, S., Hansson, L., Hernell, O., Lönnerdal, B., Nilsson, A. K., & Strömqvist, M. (1992). Cloning and sequencing of human k-casein cDNA. Mitochondrial DNA, 3(4), 245-246. https://doi.org/10.3109/10425179209034024