Cloning and sequencing of a cDNA encoding human milk β-casein

Bo Lönnerdal, Sven Bergström, Yvonne Andersson, Karin Hjalmarsson, Anna Karin Sundqvist, Olle Hemell

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A cDNA of 1065 bp encoding the human milk β-casein was cloned and sequenced using a synthetic oligodeoxyribonucleotide probe and a human mammary gland library. The nucleotide (nt) sequence contained an open reading frame sufficient to encode the entire amino-acid (aa) sequence of a β-casein precursor protein consisting of 210 aa and a signal peptide of 15 aa. The nt sequence shows 45-62% homology to those of bovine, ovine, rat, and mouse β-caseins. The highly phosphorylated site, which is responsible for the calcium-binding capacity of β-casein, the signal peptide, and a sequence encoding for an inhibitor to the angiotensin-converting enzyme seem highly conserved among the β-caseins with known sequences.

Original languageEnglish (US)
Pages (from-to)153-156
Number of pages4
JournalFEBS Letters
Volume269
Issue number1
DOIs
StatePublished - Aug 20 1990

Fingerprint

Cloning
Human Milk
Caseins
Organism Cloning
Complementary DNA
Protein Sorting Signals
Amino Acids
Nucleotides
Protein Precursors
Oligonucleotide Probes
Peptidyl-Dipeptidase A
Human Mammary Glands
Angiotensin-Converting Enzyme Inhibitors
Open Reading Frames
Rats
Amino Acid Sequence
Sheep
Calcium

Keywords

  • Angiotensin-converting enzyme inhibitor
  • Calcium-binding site
  • Human milk protein
  • Nucleotide sequence
  • Sequence comparison

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Lönnerdal, B., Bergström, S., Andersson, Y., Hjalmarsson, K., Sundqvist, A. K., & Hemell, O. (1990). Cloning and sequencing of a cDNA encoding human milk β-casein. FEBS Letters, 269(1), 153-156. https://doi.org/10.1016/0014-5793(90)81142-B

Cloning and sequencing of a cDNA encoding human milk β-casein. / Lönnerdal, Bo; Bergström, Sven; Andersson, Yvonne; Hjalmarsson, Karin; Sundqvist, Anna Karin; Hemell, Olle.

In: FEBS Letters, Vol. 269, No. 1, 20.08.1990, p. 153-156.

Research output: Contribution to journalArticle

Lönnerdal, B, Bergström, S, Andersson, Y, Hjalmarsson, K, Sundqvist, AK & Hemell, O 1990, 'Cloning and sequencing of a cDNA encoding human milk β-casein', FEBS Letters, vol. 269, no. 1, pp. 153-156. https://doi.org/10.1016/0014-5793(90)81142-B
Lönnerdal B, Bergström S, Andersson Y, Hjalmarsson K, Sundqvist AK, Hemell O. Cloning and sequencing of a cDNA encoding human milk β-casein. FEBS Letters. 1990 Aug 20;269(1):153-156. https://doi.org/10.1016/0014-5793(90)81142-B
Lönnerdal, Bo ; Bergström, Sven ; Andersson, Yvonne ; Hjalmarsson, Karin ; Sundqvist, Anna Karin ; Hemell, Olle. / Cloning and sequencing of a cDNA encoding human milk β-casein. In: FEBS Letters. 1990 ; Vol. 269, No. 1. pp. 153-156.
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