Cloning and sequence analysis of the hemagglutinin gene of the virulent strain of rinderpest virus

Miles Yamanaka, David Hsu, Tracy Crisp, Beverly Dale, Marvin Grubman, Tilahun Yilma

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We have cloned the cDNA of the hemagglutinin (HA) gene of the virulent (Kabete O) strain of rinderpest virus and produced a comparative analysis of its sequence with that of the HA genes of rinderpest (lapinized strain) and measles viruses. The gene has an open reading frame of 1827 nucleotides, and the derived protein is 609 amino acids long with a calculated molecular weight of 68,006. The Kabete O HA polypeptide is identical in length to the HA of the lapinized strain of rinderpest virus and has a similar hydropathy profile. The nucleotide divergence between the lapinized and the Kabete O HA genes is 11.4% within the coding region, and 34.3% in the 3′ untranslated region. The two rinderpest HA polypeptides differ at 74 amino acid residues for a divergence of 12.2%. Three-way comparison of the two rinderpest HA molecules with the measles virus HA polypeptide indicates that 56.6% of the residues are conserved.

Original languageEnglish (US)
Pages (from-to)251-253
Number of pages3
JournalVirology
Volume166
Issue number1
DOIs
StatePublished - 1988

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Rinderpest virus
Hemagglutinins
Sequence Analysis
Organism Cloning
Rinderpest
Genes
Measles virus
Peptides
Nucleotides
Amino Acids
3' Untranslated Regions
Open Reading Frames
Complementary DNA
Molecular Weight

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Cloning and sequence analysis of the hemagglutinin gene of the virulent strain of rinderpest virus. / Yamanaka, Miles; Hsu, David; Crisp, Tracy; Dale, Beverly; Grubman, Marvin; Yilma, Tilahun.

In: Virology, Vol. 166, No. 1, 1988, p. 251-253.

Research output: Contribution to journalArticle

Yamanaka, M, Hsu, D, Crisp, T, Dale, B, Grubman, M & Yilma, T 1988, 'Cloning and sequence analysis of the hemagglutinin gene of the virulent strain of rinderpest virus', Virology, vol. 166, no. 1, pp. 251-253. https://doi.org/10.1016/0042-6822(88)90168-7
Yamanaka, Miles ; Hsu, David ; Crisp, Tracy ; Dale, Beverly ; Grubman, Marvin ; Yilma, Tilahun. / Cloning and sequence analysis of the hemagglutinin gene of the virulent strain of rinderpest virus. In: Virology. 1988 ; Vol. 166, No. 1. pp. 251-253.
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