Cloning and nucleotide sequence of mouse immunoglobulin ε chain cDNA

Fu-Tong Liu, K. Albrandt, J. G. Sutcliffe, D. H. Katz

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

cDNA corresponding to mouse IgE heavy (ε) chain mRNA was cloned from mouse IgE-secreting hybridoma cells. A clone containing the ε cDNA insert was identified by hybridization to ε mRNA and subsequent translation in vitro to unprocessed ε chain reactive with anti-mouse IgE antibodies. This clone was used to select 20 other ε cDNA clones by colony hybridization. The clone containing the longest insert was selected and the ε cDNA insert was subjected to sequence analysis. The determined sequence is 1,279 nucleotides long and contains the coding regions for part of the constant region (C[ε]) 1 and all of the C[ε]2, C[ε]3, and C[ε]4 domains and also the entire 3' untranslated region of ε mRNA. When the amino acid sequence determined from the nucleotide sequence is compared to that of human ε chain, significant homologies between corresponding domains of the two ε chains are found, including conservations in cysteine and tryptophan residues and carbohydrate attachment sites.

Original languageEnglish (US)
Pages (from-to)7852-7856
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume79
Issue number24 I
StatePublished - 1982

Fingerprint

Immunoglobulin Subunits
Organism Cloning
Complementary DNA
Clone Cells
Immunoglobulin E
Messenger RNA
Hybridomas
Protein Biosynthesis
3' Untranslated Regions
Tryptophan
Cysteine
Sequence Analysis
Amino Acid Sequence
Nucleotides
Carbohydrates

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Cloning and nucleotide sequence of mouse immunoglobulin ε chain cDNA. / Liu, Fu-Tong; Albrandt, K.; Sutcliffe, J. G.; Katz, D. H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 79, No. 24 I, 1982, p. 7852-7856.

Research output: Contribution to journalArticle

Liu, Fu-Tong ; Albrandt, K. ; Sutcliffe, J. G. ; Katz, D. H. / Cloning and nucleotide sequence of mouse immunoglobulin ε chain cDNA. In: Proceedings of the National Academy of Sciences of the United States of America. 1982 ; Vol. 79, No. 24 I. pp. 7852-7856.
@article{80d03a82998d4252a5a9b64f639e3a8f,
title = "Cloning and nucleotide sequence of mouse immunoglobulin ε chain cDNA",
abstract = "cDNA corresponding to mouse IgE heavy (ε) chain mRNA was cloned from mouse IgE-secreting hybridoma cells. A clone containing the ε cDNA insert was identified by hybridization to ε mRNA and subsequent translation in vitro to unprocessed ε chain reactive with anti-mouse IgE antibodies. This clone was used to select 20 other ε cDNA clones by colony hybridization. The clone containing the longest insert was selected and the ε cDNA insert was subjected to sequence analysis. The determined sequence is 1,279 nucleotides long and contains the coding regions for part of the constant region (C[ε]) 1 and all of the C[ε]2, C[ε]3, and C[ε]4 domains and also the entire 3' untranslated region of ε mRNA. When the amino acid sequence determined from the nucleotide sequence is compared to that of human ε chain, significant homologies between corresponding domains of the two ε chains are found, including conservations in cysteine and tryptophan residues and carbohydrate attachment sites.",
author = "Fu-Tong Liu and K. Albrandt and Sutcliffe, {J. G.} and Katz, {D. H.}",
year = "1982",
language = "English (US)",
volume = "79",
pages = "7852--7856",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "24 I",

}

TY - JOUR

T1 - Cloning and nucleotide sequence of mouse immunoglobulin ε chain cDNA

AU - Liu, Fu-Tong

AU - Albrandt, K.

AU - Sutcliffe, J. G.

AU - Katz, D. H.

PY - 1982

Y1 - 1982

N2 - cDNA corresponding to mouse IgE heavy (ε) chain mRNA was cloned from mouse IgE-secreting hybridoma cells. A clone containing the ε cDNA insert was identified by hybridization to ε mRNA and subsequent translation in vitro to unprocessed ε chain reactive with anti-mouse IgE antibodies. This clone was used to select 20 other ε cDNA clones by colony hybridization. The clone containing the longest insert was selected and the ε cDNA insert was subjected to sequence analysis. The determined sequence is 1,279 nucleotides long and contains the coding regions for part of the constant region (C[ε]) 1 and all of the C[ε]2, C[ε]3, and C[ε]4 domains and also the entire 3' untranslated region of ε mRNA. When the amino acid sequence determined from the nucleotide sequence is compared to that of human ε chain, significant homologies between corresponding domains of the two ε chains are found, including conservations in cysteine and tryptophan residues and carbohydrate attachment sites.

AB - cDNA corresponding to mouse IgE heavy (ε) chain mRNA was cloned from mouse IgE-secreting hybridoma cells. A clone containing the ε cDNA insert was identified by hybridization to ε mRNA and subsequent translation in vitro to unprocessed ε chain reactive with anti-mouse IgE antibodies. This clone was used to select 20 other ε cDNA clones by colony hybridization. The clone containing the longest insert was selected and the ε cDNA insert was subjected to sequence analysis. The determined sequence is 1,279 nucleotides long and contains the coding regions for part of the constant region (C[ε]) 1 and all of the C[ε]2, C[ε]3, and C[ε]4 domains and also the entire 3' untranslated region of ε mRNA. When the amino acid sequence determined from the nucleotide sequence is compared to that of human ε chain, significant homologies between corresponding domains of the two ε chains are found, including conservations in cysteine and tryptophan residues and carbohydrate attachment sites.

UR - http://www.scopus.com/inward/record.url?scp=0020460419&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020460419&partnerID=8YFLogxK

M3 - Article

VL - 79

SP - 7852

EP - 7856

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 24 I

ER -