Cloning and nucleotide sequence of mouse immunoglobulin ε chain cDNA

Fu-Tong Liu, K. Albrandt, J. G. Sutcliffe, D. H. Katz

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

cDNA corresponding to mouse IgE heavy (ε) chain mRNA was cloned from mouse IgE-secreting hybridoma cells. A clone containing the ε cDNA insert was identified by hybridization to ε mRNA and subsequent translation in vitro to unprocessed ε chain reactive with anti-mouse IgE antibodies. This clone was used to select 20 other ε cDNA clones by colony hybridization. The clone containing the longest insert was selected and the ε cDNA insert was subjected to sequence analysis. The determined sequence is 1,279 nucleotides long and contains the coding regions for part of the constant region (C[ε]) 1 and all of the C[ε]2, C[ε]3, and C[ε]4 domains and also the entire 3' untranslated region of ε mRNA. When the amino acid sequence determined from the nucleotide sequence is compared to that of human ε chain, significant homologies between corresponding domains of the two ε chains are found, including conservations in cysteine and tryptophan residues and carbohydrate attachment sites.

Original languageEnglish (US)
Pages (from-to)7852-7856
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume79
Issue number24 I
StatePublished - 1982

ASJC Scopus subject areas

  • General
  • Genetics

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