Cloning and functional characterization of allelic variation in the porcine prolactin receptor

Josephine F. Trott, Nicholas R. Farley, Douglas J. Taatjes, Russell C. Hovey

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Prolactin (PRL) regulates various functions in pigs including reproduction, mammary development and lactation. We used 5′-rapid amplification of cDNA ends (5′-RACE) to clone three full-length alleles of the porcine PRL receptor (pPRLR) from Landrace (alleles LR2 and LR4) and Yucatan miniature (MP) pigs, corresponding to the A and B alleles previously reported to be associated with reproductive traits. When expressed in Chinese hamster ovary (CHO-K1) cells, all three pPRLRs transduced differentiation signals to a β-casein promoter with the same effectiveness, where human growth hormone (hGH) and porcine PRL (pPRL) were more effective ligands than ovine PRL (oPRL). The pPRLR had a lower binding affinity for oPRL than pPRL while binding affinity for hGH was not different between the three pPRLR variants. The pPRLRs primarily localized to the cytoplasm with perinuclear concentration. In conclusion, we have cloned three allelic variants of the pPRLR and have functionally characterized these as different from the hPRLR. However, our data do not support the proposal that allelic variation of the pPRLR confers functional differences in vivo.

Original languageEnglish (US)
Pages (from-to)313-334
Number of pages22
JournalDomestic Animal Endocrinology
Issue number3
StatePublished - Oct 2007
Externally publishedYes


  • Polymorphism
  • Porcine
  • Prolactin receptor
  • Reproduction
  • Tissue distribution

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)
  • Endocrinology


Dive into the research topics of 'Cloning and functional characterization of allelic variation in the porcine prolactin receptor'. Together they form a unique fingerprint.

Cite this