Cloning and function of sanJ-A gene involved in nikkomycins biosynthesis of Streptomyces ansochromogenes

Li Ping Nie, Yu Qing Tian, Hua Rong Tan

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A 7.5kb DNA fragment was identified from the cosmid library of Streptomyces ansochromogenesus using a partial DNA fragment involved in the nikkomycin biosynthesis as a probe. It was cloned into pBluescript M13- and the resulting recombinant plasmid was designated as pNL2200. The nucleotide sequence of 2.3kb Sal I -BamH I DNA fragment was determined and analysed. The result indicated that the fragment contains one complete open reading frame (ORF); The start codon is GTG at 271 position and the stop codon is TGA at 1 954 position. It contains 1 686 base pairs and encodes a protein with 561 amino acids. The deduced product has 44% identity with the ligase belong to the superfamily of adenylate-forming enzyme. The experiment of gene disruption showed that the sanJ gene was closely related to nikkomycins biosynthesis of Streptomyces ansochromogenes. It was designated as sanJ.

Original languageEnglish (US)
Pages (from-to)194-195
Number of pages2
JournalActa Genetica Sinica
Volume28
Issue number2
StatePublished - 2001
Externally publishedYes

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Keywords

  • Adenylate-forming enzyme
  • Biosynthesis
  • Nikkomycins
  • Streptomyces ansochromogene

ASJC Scopus subject areas

  • Genetics

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