Abstract
A 7.5kb DNA fragment was identified from the cosmid library of Streptomyces ansochromogenesus using a partial DNA fragment involved in the nikkomycin biosynthesis as a probe. It was cloned into pBluescript M13- and the resulting recombinant plasmid was designated as pNL2200. The nucleotide sequence of 2.3kb Sal I -BamH I DNA fragment was determined and analysed. The result indicated that the fragment contains one complete open reading frame (ORF); The start codon is GTG at 271 position and the stop codon is TGA at 1 954 position. It contains 1 686 base pairs and encodes a protein with 561 amino acids. The deduced product has 44% identity with the ligase belong to the superfamily of adenylate-forming enzyme. The experiment of gene disruption showed that the sanJ gene was closely related to nikkomycins biosynthesis of Streptomyces ansochromogenes. It was designated as sanJ.
Original language | English (US) |
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Pages (from-to) | 194-195 |
Number of pages | 2 |
Journal | Acta Genetica Sinica |
Volume | 28 |
Issue number | 2 |
State | Published - 2001 |
Externally published | Yes |
Keywords
- Adenylate-forming enzyme
- Biosynthesis
- Nikkomycins
- Streptomyces ansochromogene
ASJC Scopus subject areas
- Genetics