Abstract
A 7.5kb DNA fragment was identified from the cosmid library of Streptomyces ansochromogenesus using a partial DNA fragment involved in the nikkomycin biosynthesis as a probe. It was cloned into pBluescript M13- and the resulting recombinant plasmid was designated as pNL2200. The nucleotide sequence of 2.3kb Sal I -BamH I DNA fragment was determined and analysed. The result indicated that the fragment contains one complete open reading frame (ORF); The start codon is GTG at 271 position and the stop codon is TGA at 1 954 position. It contains 1 686 base pairs and encodes a protein with 561 amino acids. The deduced product has 44% identity with the ligase belong to the superfamily of adenylate-forming enzyme. The experiment of gene disruption showed that the sanJ gene was closely related to nikkomycins biosynthesis of Streptomyces ansochromogenes. It was designated as sanJ.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 194-195 |
| Number of pages | 2 |
| Journal | Acta Genetica Sinica |
| Volume | 28 |
| Issue number | 2 |
| State | Published - 2001 |
| Externally published | Yes |
Fingerprint
Keywords
- Adenylate-forming enzyme
- Biosynthesis
- Nikkomycins
- Streptomyces ansochromogene
ASJC Scopus subject areas
- Genetics
Cite this
Cloning and function of sanJ-A gene involved in nikkomycins biosynthesis of Streptomyces ansochromogenes. / Nie, Li Ping; Tian, Yu Qing; Tan, Hua Rong.
In: Acta Genetica Sinica, Vol. 28, No. 2, 2001, p. 194-195.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cloning and function of sanJ-A gene involved in nikkomycins biosynthesis of Streptomyces ansochromogenes
AU - Nie, Li Ping
AU - Tian, Yu Qing
AU - Tan, Hua Rong
PY - 2001
Y1 - 2001
N2 - A 7.5kb DNA fragment was identified from the cosmid library of Streptomyces ansochromogenesus using a partial DNA fragment involved in the nikkomycin biosynthesis as a probe. It was cloned into pBluescript M13- and the resulting recombinant plasmid was designated as pNL2200. The nucleotide sequence of 2.3kb Sal I -BamH I DNA fragment was determined and analysed. The result indicated that the fragment contains one complete open reading frame (ORF); The start codon is GTG at 271 position and the stop codon is TGA at 1 954 position. It contains 1 686 base pairs and encodes a protein with 561 amino acids. The deduced product has 44% identity with the ligase belong to the superfamily of adenylate-forming enzyme. The experiment of gene disruption showed that the sanJ gene was closely related to nikkomycins biosynthesis of Streptomyces ansochromogenes. It was designated as sanJ.
AB - A 7.5kb DNA fragment was identified from the cosmid library of Streptomyces ansochromogenesus using a partial DNA fragment involved in the nikkomycin biosynthesis as a probe. It was cloned into pBluescript M13- and the resulting recombinant plasmid was designated as pNL2200. The nucleotide sequence of 2.3kb Sal I -BamH I DNA fragment was determined and analysed. The result indicated that the fragment contains one complete open reading frame (ORF); The start codon is GTG at 271 position and the stop codon is TGA at 1 954 position. It contains 1 686 base pairs and encodes a protein with 561 amino acids. The deduced product has 44% identity with the ligase belong to the superfamily of adenylate-forming enzyme. The experiment of gene disruption showed that the sanJ gene was closely related to nikkomycins biosynthesis of Streptomyces ansochromogenes. It was designated as sanJ.
KW - Adenylate-forming enzyme
KW - Biosynthesis
KW - Nikkomycins
KW - Streptomyces ansochromogene
UR - http://www.scopus.com/inward/record.url?scp=0035228160&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035228160&partnerID=8YFLogxK
M3 - Article
VL - 28
SP - 194
EP - 195
JO - Journal of Genetics and Genomics
JF - Journal of Genetics and Genomics
SN - 1673-8527
IS - 2
ER -