Abstract
Peste des petits ruminants (PPR) is a viral disease of goats and sheep characterized by erosive stomatitis, enteriris, and pneumonia. The virus is a member of the family Paramyxoviridae and the genus Morbillivirus. The disease has high morbidity and mortality rates and has a substantial economic impact in developing countries. We have cloned and sequenced the cDNA of the nucleocapsid (N) gene of the Nigeria 75/1 strain of PPR virus (PPRV). A comparison of its nucleotide and deduced amino acid sequence with those of the N gene of the tissue culture-attenuated strain of PPRV was performed. A divergence of 8.9 and 5.0% was found at the nucleotide and amino acid level, respectively. A recombinant baculovirus that expresses the N protein in insect cells and larvae (Spodoptera frugiperda) was generated. The recombinant protein, characterized by Western blot analysis, was shown to have a molecular weight of 58 kDa and was recognized by anti-PPRV antibodies. The recombinant protein was used successfully as a coating antigen in an enzyme-linked immunosorbent assay for the serological diagnosis of PPRV.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 776-778 |
| Number of pages | 3 |
| Journal | Virology |
| Volume | 208 |
| Issue number | 2 |
| DOIs | |
| State | Published - Apr 20 1995 |
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ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
Cloning and Expression of the Nucleoprotein of Peste des Petits Ruminants Virus in Baculovirus for Use in Serological Diagnosis. / Ismail, Tarek M.; Yamanaka, Miles K.; Saliki, Jeremiah T.; El-Kholy, Alaa; Mebus, Charles; Yilma, Tilahun.
In: Virology, Vol. 208, No. 2, 20.04.1995, p. 776-778.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Cloning and Expression of the Nucleoprotein of Peste des Petits Ruminants Virus in Baculovirus for Use in Serological Diagnosis
AU - Ismail, Tarek M.
AU - Yamanaka, Miles K.
AU - Saliki, Jeremiah T.
AU - El-Kholy, Alaa
AU - Mebus, Charles
AU - Yilma, Tilahun
PY - 1995/4/20
Y1 - 1995/4/20
N2 - Peste des petits ruminants (PPR) is a viral disease of goats and sheep characterized by erosive stomatitis, enteriris, and pneumonia. The virus is a member of the family Paramyxoviridae and the genus Morbillivirus. The disease has high morbidity and mortality rates and has a substantial economic impact in developing countries. We have cloned and sequenced the cDNA of the nucleocapsid (N) gene of the Nigeria 75/1 strain of PPR virus (PPRV). A comparison of its nucleotide and deduced amino acid sequence with those of the N gene of the tissue culture-attenuated strain of PPRV was performed. A divergence of 8.9 and 5.0% was found at the nucleotide and amino acid level, respectively. A recombinant baculovirus that expresses the N protein in insect cells and larvae (Spodoptera frugiperda) was generated. The recombinant protein, characterized by Western blot analysis, was shown to have a molecular weight of 58 kDa and was recognized by anti-PPRV antibodies. The recombinant protein was used successfully as a coating antigen in an enzyme-linked immunosorbent assay for the serological diagnosis of PPRV.
AB - Peste des petits ruminants (PPR) is a viral disease of goats and sheep characterized by erosive stomatitis, enteriris, and pneumonia. The virus is a member of the family Paramyxoviridae and the genus Morbillivirus. The disease has high morbidity and mortality rates and has a substantial economic impact in developing countries. We have cloned and sequenced the cDNA of the nucleocapsid (N) gene of the Nigeria 75/1 strain of PPR virus (PPRV). A comparison of its nucleotide and deduced amino acid sequence with those of the N gene of the tissue culture-attenuated strain of PPRV was performed. A divergence of 8.9 and 5.0% was found at the nucleotide and amino acid level, respectively. A recombinant baculovirus that expresses the N protein in insect cells and larvae (Spodoptera frugiperda) was generated. The recombinant protein, characterized by Western blot analysis, was shown to have a molecular weight of 58 kDa and was recognized by anti-PPRV antibodies. The recombinant protein was used successfully as a coating antigen in an enzyme-linked immunosorbent assay for the serological diagnosis of PPRV.
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U2 - 10.1006/viro.1995.1210
DO - 10.1006/viro.1995.1210
M3 - Article
VL - 208
SP - 776
EP - 778
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -