Cloning and Expression of the Nucleoprotein of Peste des Petits Ruminants Virus in Baculovirus for Use in Serological Diagnosis

Tarek M. Ismail, Miles K. Yamanaka, Jeremiah T. Saliki, Alaa El-Kholy, Charles Mebus, Tilahun Yilma

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Peste des petits ruminants (PPR) is a viral disease of goats and sheep characterized by erosive stomatitis, enteriris, and pneumonia. The virus is a member of the family Paramyxoviridae and the genus Morbillivirus. The disease has high morbidity and mortality rates and has a substantial economic impact in developing countries. We have cloned and sequenced the cDNA of the nucleocapsid (N) gene of the Nigeria 75/1 strain of PPR virus (PPRV). A comparison of its nucleotide and deduced amino acid sequence with those of the N gene of the tissue culture-attenuated strain of PPRV was performed. A divergence of 8.9 and 5.0% was found at the nucleotide and amino acid level, respectively. A recombinant baculovirus that expresses the N protein in insect cells and larvae (Spodoptera frugiperda) was generated. The recombinant protein, characterized by Western blot analysis, was shown to have a molecular weight of 58 kDa and was recognized by anti-PPRV antibodies. The recombinant protein was used successfully as a coating antigen in an enzyme-linked immunosorbent assay for the serological diagnosis of PPRV.

Original languageEnglish (US)
Pages (from-to)776-778
Number of pages3
JournalVirology
Volume208
Issue number2
DOIs
StatePublished - Apr 20 1995

Fingerprint

Peste-des-petits-ruminants virus
Nucleoproteins
Baculoviridae
Organism Cloning
Viruses
Recombinant Proteins
Peste-des-Petits-Ruminants
Paramyxoviridae
Morbillivirus
Nucleotides
Insect Proteins
Spodoptera
Nucleocapsid
Stomatitis
Virus Diseases
Nigeria
Goats
Developing Countries
Genes
Larva

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Cloning and Expression of the Nucleoprotein of Peste des Petits Ruminants Virus in Baculovirus for Use in Serological Diagnosis. / Ismail, Tarek M.; Yamanaka, Miles K.; Saliki, Jeremiah T.; El-Kholy, Alaa; Mebus, Charles; Yilma, Tilahun.

In: Virology, Vol. 208, No. 2, 20.04.1995, p. 776-778.

Research output: Contribution to journalArticle

Ismail, Tarek M. ; Yamanaka, Miles K. ; Saliki, Jeremiah T. ; El-Kholy, Alaa ; Mebus, Charles ; Yilma, Tilahun. / Cloning and Expression of the Nucleoprotein of Peste des Petits Ruminants Virus in Baculovirus for Use in Serological Diagnosis. In: Virology. 1995 ; Vol. 208, No. 2. pp. 776-778.
@article{8c1d9e01cc4f43338e97c1918eac33ce,
title = "Cloning and Expression of the Nucleoprotein of Peste des Petits Ruminants Virus in Baculovirus for Use in Serological Diagnosis",
abstract = "Peste des petits ruminants (PPR) is a viral disease of goats and sheep characterized by erosive stomatitis, enteriris, and pneumonia. The virus is a member of the family Paramyxoviridae and the genus Morbillivirus. The disease has high morbidity and mortality rates and has a substantial economic impact in developing countries. We have cloned and sequenced the cDNA of the nucleocapsid (N) gene of the Nigeria 75/1 strain of PPR virus (PPRV). A comparison of its nucleotide and deduced amino acid sequence with those of the N gene of the tissue culture-attenuated strain of PPRV was performed. A divergence of 8.9 and 5.0{\%} was found at the nucleotide and amino acid level, respectively. A recombinant baculovirus that expresses the N protein in insect cells and larvae (Spodoptera frugiperda) was generated. The recombinant protein, characterized by Western blot analysis, was shown to have a molecular weight of 58 kDa and was recognized by anti-PPRV antibodies. The recombinant protein was used successfully as a coating antigen in an enzyme-linked immunosorbent assay for the serological diagnosis of PPRV.",
author = "Ismail, {Tarek M.} and Yamanaka, {Miles K.} and Saliki, {Jeremiah T.} and Alaa El-Kholy and Charles Mebus and Tilahun Yilma",
year = "1995",
month = "4",
day = "20",
doi = "10.1006/viro.1995.1210",
language = "English (US)",
volume = "208",
pages = "776--778",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Cloning and Expression of the Nucleoprotein of Peste des Petits Ruminants Virus in Baculovirus for Use in Serological Diagnosis

AU - Ismail, Tarek M.

AU - Yamanaka, Miles K.

AU - Saliki, Jeremiah T.

AU - El-Kholy, Alaa

AU - Mebus, Charles

AU - Yilma, Tilahun

PY - 1995/4/20

Y1 - 1995/4/20

N2 - Peste des petits ruminants (PPR) is a viral disease of goats and sheep characterized by erosive stomatitis, enteriris, and pneumonia. The virus is a member of the family Paramyxoviridae and the genus Morbillivirus. The disease has high morbidity and mortality rates and has a substantial economic impact in developing countries. We have cloned and sequenced the cDNA of the nucleocapsid (N) gene of the Nigeria 75/1 strain of PPR virus (PPRV). A comparison of its nucleotide and deduced amino acid sequence with those of the N gene of the tissue culture-attenuated strain of PPRV was performed. A divergence of 8.9 and 5.0% was found at the nucleotide and amino acid level, respectively. A recombinant baculovirus that expresses the N protein in insect cells and larvae (Spodoptera frugiperda) was generated. The recombinant protein, characterized by Western blot analysis, was shown to have a molecular weight of 58 kDa and was recognized by anti-PPRV antibodies. The recombinant protein was used successfully as a coating antigen in an enzyme-linked immunosorbent assay for the serological diagnosis of PPRV.

AB - Peste des petits ruminants (PPR) is a viral disease of goats and sheep characterized by erosive stomatitis, enteriris, and pneumonia. The virus is a member of the family Paramyxoviridae and the genus Morbillivirus. The disease has high morbidity and mortality rates and has a substantial economic impact in developing countries. We have cloned and sequenced the cDNA of the nucleocapsid (N) gene of the Nigeria 75/1 strain of PPR virus (PPRV). A comparison of its nucleotide and deduced amino acid sequence with those of the N gene of the tissue culture-attenuated strain of PPRV was performed. A divergence of 8.9 and 5.0% was found at the nucleotide and amino acid level, respectively. A recombinant baculovirus that expresses the N protein in insect cells and larvae (Spodoptera frugiperda) was generated. The recombinant protein, characterized by Western blot analysis, was shown to have a molecular weight of 58 kDa and was recognized by anti-PPRV antibodies. The recombinant protein was used successfully as a coating antigen in an enzyme-linked immunosorbent assay for the serological diagnosis of PPRV.

UR - http://www.scopus.com/inward/record.url?scp=0029037296&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029037296&partnerID=8YFLogxK

U2 - 10.1006/viro.1995.1210

DO - 10.1006/viro.1995.1210

M3 - Article

C2 - 7747450

AN - SCOPUS:0029037296

VL - 208

SP - 776

EP - 778

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -