Cloning and expression of cDNAs for the α subunit of the murine lymphocyte-Peyer's patch adhesion molecule

Heinz Neuhaus, Mickey C T Hu, Martin E. Hemler, Yoshikazu Takada, Bernhard Holzmann, Irving L. Weissman

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

cDNA clones encoding the α chain of the murine lymphocyte-Peyer's patch adhesion molecule (LPAM), which is associated with lymphocyte homing, have been isolated by screening with the human VLA-4 (α4h) probe. Several α4 antigenic determinants were identified on COS-7 cells after transfection. From overlapping clones, ∼5 kb of contiguous nucleotide sequence have been determined, encoding a protein sequence of 1039 amino acids for the LPAM α chain (α4m). LPAM is a member of the integrin family of cell-surface heterodimers, and α4m is the murine homologue of the human α4h chain. The two proteins have a total sequence similarity of 84%, with an almost perfect conservation (31/32 amino acids) in the cytoplasmic domain. Like α4h, α4m is distinct from other integrin α chains because it has neither an I-domain nor a COOH-terminal cleavage site. The positions of the characteristic Cysteine residues are conserved, and a putative protease cleavage site is located near the middle of the protein sequence. The NH2-terminal part of the protein contains seven homologous repeats, and three of them include putative divalent cation-binding sites. These sites are among the most conserved between the α4m sequence and other α chains, and may therefore be involved in the binding of integrin α and β chains. An additional cDNA clone was isolated which shares a sequence of perfect homology with the α4m encoding cDNAs, but has a unique 3′ poly-A end. This observation correlates with the fact that three discrete murine RNA bands are observed in Northern blot experiments using α4m as a probe, whereas only two human RNA species are described for α4h, indicating a higher complexity for murine than for human sequences.

Original languageEnglish (US)
Pages (from-to)1149-1158
Number of pages10
JournalJournal of Cell Biology
Volume115
Issue number4
StatePublished - Nov 1991
Externally publishedYes

Fingerprint

Organism Cloning
Complementary DNA
Integrins
Clone Cells
Proteins
RNA
Integrin alpha4beta1
Poly A
COS Cells
Divalent Cations
Sequence Homology
Northern Blotting
Transfection
Cysteine
Epitopes
Amino Acid Sequence
Peptide Hydrolases
Binding Sites
Lymphocytes
Amino Acids

ASJC Scopus subject areas

  • Cell Biology

Cite this

Neuhaus, H., Hu, M. C. T., Hemler, M. E., Takada, Y., Holzmann, B., & Weissman, I. L. (1991). Cloning and expression of cDNAs for the α subunit of the murine lymphocyte-Peyer's patch adhesion molecule. Journal of Cell Biology, 115(4), 1149-1158.

Cloning and expression of cDNAs for the α subunit of the murine lymphocyte-Peyer's patch adhesion molecule. / Neuhaus, Heinz; Hu, Mickey C T; Hemler, Martin E.; Takada, Yoshikazu; Holzmann, Bernhard; Weissman, Irving L.

In: Journal of Cell Biology, Vol. 115, No. 4, 11.1991, p. 1149-1158.

Research output: Contribution to journalArticle

Neuhaus, H, Hu, MCT, Hemler, ME, Takada, Y, Holzmann, B & Weissman, IL 1991, 'Cloning and expression of cDNAs for the α subunit of the murine lymphocyte-Peyer's patch adhesion molecule', Journal of Cell Biology, vol. 115, no. 4, pp. 1149-1158.
Neuhaus, Heinz ; Hu, Mickey C T ; Hemler, Martin E. ; Takada, Yoshikazu ; Holzmann, Bernhard ; Weissman, Irving L. / Cloning and expression of cDNAs for the α subunit of the murine lymphocyte-Peyer's patch adhesion molecule. In: Journal of Cell Biology. 1991 ; Vol. 115, No. 4. pp. 1149-1158.
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