Cloning and comparative analysis of carotenoid β-hydroxylase genes provides new insights into carotenoid metabolism in tetraploid (Triticum turgidum ssp. durum) and hexaploid (Triticum aestivum) wheat grains

Xiaoqiong Qin, Wenjun Zhang, Jorge Dubcovsky, Li Tian

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Carotenoid β-hydroxylases attach hydroxyl groups to the β-ionone rings (β-rings) of carotenoid substrates, resulting in modified structures and functions of carotenoid molecules. We cloned and characterized two genes (each with three homeologs), HYD1 and HYD2, which encode β-hydroxylases in wheat. The results from bioinformatic and nested degenerate PCR analyses collectively suggest that HYD1 and HYD2 may represent the entire complement of non-heme di-iron β-hydroxylases in wheat. The homeologs of wheat HYDs exhibited major β-ring and minor ε-ring hydroxylation activities in carotenoid-accumulating E. coli strains. Distinct expression patterns were observed for different HYD genes and homeologs in vegetative tissues and developing grains of tetraploid and hexaploid wheat, suggesting their functional divergence and differential regulatory control in tissue-, grain development-, and ploidy-specific manners. An intriguing observation was that the expression of HYD1, particularly HYD-B1, reached highest levels at the last stage of tetraploid and hexaploid grain development, suggesting that carotenoids (at least xanthophylls) were still actively synthesized in mature grains. This result challenges the common perception that carotenoids are simply being turned over during wheat grain development after their initial biosynthesis at the early grain development stages. Overall, this improved understanding of carotenoid biosynthetic gene expression and carotenoid metabolism in wheat grains will contribute to the improvement of the nutritional value of wheat grains for human consumption.

Original languageEnglish (US)
Pages (from-to)631-646
Number of pages16
JournalPlant Molecular Biology
Volume80
Issue number6
DOIs
StatePublished - Dec 2012

Fingerprint

Tetraploidy
Triticum turgidum subsp. durum
Carotenoids
hexaploidy
Mixed Function Oxygenases
tetraploidy
Triticum
Organism Cloning
molecular cloning
carotenoids
Triticum aestivum
wheat
metabolism
seed development
Genes
genes
Norisoprenoids
Xanthophylls
norisoprenoids
xanthophylls

Keywords

  • β-hydroxylase
  • Carotenoid
  • Homeolog
  • Lutein
  • Provitamin A
  • Wheat

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science
  • Genetics

Cite this

Cloning and comparative analysis of carotenoid β-hydroxylase genes provides new insights into carotenoid metabolism in tetraploid (Triticum turgidum ssp. durum) and hexaploid (Triticum aestivum) wheat grains. / Qin, Xiaoqiong; Zhang, Wenjun; Dubcovsky, Jorge; Tian, Li.

In: Plant Molecular Biology, Vol. 80, No. 6, 12.2012, p. 631-646.

Research output: Contribution to journalArticle

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