Cloning and characterization of cDNAs for two distinct tumor necrosis factor receptor superfamily genes from Japanese flounder Paralichthys olivaceus

Chan Il Park, Tomofumi Kurobe, Ikuo Hirono, Takashi Aoki

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

Tumor necrosis factor receptor (TNFR) superfamily regulates diverse biologic functions, including cell proliferation, differentiation, and survival, in addition to providing costimulatory signals for programmed cell death or apoptosis. In this study, cDNA fragments for two distinct TNFR homologues were obtained from a Japanese flounder, Paralichthys olivaceus, cDNA library. Full-length cDNAs of TNFR-1 and TNFR-2 homologues were obtained by using these cDNA fragments as probes. The cDNA for the Japanese flounder TNFR-1 homologue predicts a peptide of 395 amino acids that is 35% identical to the extracellular region of mouse TNFR-1, whereas the cDNA of the Japanese flounder TNFR-2 homologue predicts a peptide of 483 amino acids that is 40% identical to the extracellular region of human TNFR-2. The cytoplasmic domain contains a sequence that has the consensus motif of the death domain of the Japanese flounder TNFR-1 homologue. In a healthy fish, the mRNAs of both TNFR homologues were predominantly expressed in leukocytes, kidney, gill, and spleen. Expression of the Japanese flounder TNFR-1 homologue was induced in peripheral blood lymphocytes (PBLs) after stimulation with LPS (500μg/ml) for 1h, and TNFR-2 homologue was strongly induced in PBLs after stimulation with Con A (50μg/ml) and PMA (0.35μg/ml) for 3h. The different expression patterns of the two distinct TNFR homologues may be critical in determining whether binding with TNF-α or TNF-β have activating, proliferative, or apoptotic effects on target cells.

Original languageEnglish (US)
Pages (from-to)365-375
Number of pages11
JournalDevelopmental and Comparative Immunology
Volume27
Issue number5
DOIs
StatePublished - May 1 2003
Externally publishedYes

Keywords

  • Apoptosis
  • cDNA
  • Con A/PMA
  • Japanese flounder
  • LPS
  • Peripheral blood lymphocyte
  • Tumor necrosis factor receptor

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

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