Cloning and characterization of cDNAs for two distinct tumor necrosis factor receptor superfamily genes from Japanese flounder Paralichthys olivaceus

Chan Il Park, Tomofumi Kurobe, Ikuo Hirono, Takashi Aoki

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Tumor necrosis factor receptor (TNFR) superfamily regulates diverse biologic functions, including cell proliferation, differentiation, and survival, in addition to providing costimulatory signals for programmed cell death or apoptosis. In this study, cDNA fragments for two distinct TNFR homologues were obtained from a Japanese flounder, Paralichthys olivaceus, cDNA library. Full-length cDNAs of TNFR-1 and TNFR-2 homologues were obtained by using these cDNA fragments as probes. The cDNA for the Japanese flounder TNFR-1 homologue predicts a peptide of 395 amino acids that is 35% identical to the extracellular region of mouse TNFR-1, whereas the cDNA of the Japanese flounder TNFR-2 homologue predicts a peptide of 483 amino acids that is 40% identical to the extracellular region of human TNFR-2. The cytoplasmic domain contains a sequence that has the consensus motif of the death domain of the Japanese flounder TNFR-1 homologue. In a healthy fish, the mRNAs of both TNFR homologues were predominantly expressed in leukocytes, kidney, gill, and spleen. Expression of the Japanese flounder TNFR-1 homologue was induced in peripheral blood lymphocytes (PBLs) after stimulation with LPS (500μg/ml) for 1h, and TNFR-2 homologue was strongly induced in PBLs after stimulation with Con A (50μg/ml) and PMA (0.35μg/ml) for 3h. The different expression patterns of the two distinct TNFR homologues may be critical in determining whether binding with TNF-α or TNF-β have activating, proliferative, or apoptotic effects on target cells.

Original languageEnglish (US)
Pages (from-to)365-375
Number of pages11
JournalDevelopmental and Comparative Immunology
Volume27
Issue number5
DOIs
StatePublished - May 1 2003
Externally publishedYes

Fingerprint

Flounder
Tumor Necrosis Factor Receptors
Organism Cloning
Complementary DNA
Receptors, Tumor Necrosis Factor, Type II
Genes
Lymphocyte Activation
Amino Acids
Peptides
Consensus Sequence
Gene Library
Cell Differentiation
Cell Survival
Fishes
Leukocytes
Cell Death
Spleen
Cell Proliferation
Apoptosis
Kidney

Keywords

  • Apoptosis
  • cDNA
  • Con A/PMA
  • Japanese flounder
  • LPS
  • Peripheral blood lymphocyte
  • Tumor necrosis factor receptor

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

Cite this

Cloning and characterization of cDNAs for two distinct tumor necrosis factor receptor superfamily genes from Japanese flounder Paralichthys olivaceus. / Park, Chan Il; Kurobe, Tomofumi; Hirono, Ikuo; Aoki, Takashi.

In: Developmental and Comparative Immunology, Vol. 27, No. 5, 01.05.2003, p. 365-375.

Research output: Contribution to journalArticle

@article{a77c3f1cde224795b1f39f78fb889a76,
title = "Cloning and characterization of cDNAs for two distinct tumor necrosis factor receptor superfamily genes from Japanese flounder Paralichthys olivaceus",
abstract = "Tumor necrosis factor receptor (TNFR) superfamily regulates diverse biologic functions, including cell proliferation, differentiation, and survival, in addition to providing costimulatory signals for programmed cell death or apoptosis. In this study, cDNA fragments for two distinct TNFR homologues were obtained from a Japanese flounder, Paralichthys olivaceus, cDNA library. Full-length cDNAs of TNFR-1 and TNFR-2 homologues were obtained by using these cDNA fragments as probes. The cDNA for the Japanese flounder TNFR-1 homologue predicts a peptide of 395 amino acids that is 35{\%} identical to the extracellular region of mouse TNFR-1, whereas the cDNA of the Japanese flounder TNFR-2 homologue predicts a peptide of 483 amino acids that is 40{\%} identical to the extracellular region of human TNFR-2. The cytoplasmic domain contains a sequence that has the consensus motif of the death domain of the Japanese flounder TNFR-1 homologue. In a healthy fish, the mRNAs of both TNFR homologues were predominantly expressed in leukocytes, kidney, gill, and spleen. Expression of the Japanese flounder TNFR-1 homologue was induced in peripheral blood lymphocytes (PBLs) after stimulation with LPS (500μg/ml) for 1h, and TNFR-2 homologue was strongly induced in PBLs after stimulation with Con A (50μg/ml) and PMA (0.35μg/ml) for 3h. The different expression patterns of the two distinct TNFR homologues may be critical in determining whether binding with TNF-α or TNF-β have activating, proliferative, or apoptotic effects on target cells.",
keywords = "Apoptosis, cDNA, Con A/PMA, Japanese flounder, LPS, Peripheral blood lymphocyte, Tumor necrosis factor receptor",
author = "Park, {Chan Il} and Tomofumi Kurobe and Ikuo Hirono and Takashi Aoki",
year = "2003",
month = "5",
day = "1",
doi = "10.1016/S0145-305X(02)00118-0",
language = "English (US)",
volume = "27",
pages = "365--375",
journal = "Developmental and Comparative Immunology",
issn = "0145-305X",
publisher = "Elsevier Limited",
number = "5",

}

TY - JOUR

T1 - Cloning and characterization of cDNAs for two distinct tumor necrosis factor receptor superfamily genes from Japanese flounder Paralichthys olivaceus

AU - Park, Chan Il

AU - Kurobe, Tomofumi

AU - Hirono, Ikuo

AU - Aoki, Takashi

PY - 2003/5/1

Y1 - 2003/5/1

N2 - Tumor necrosis factor receptor (TNFR) superfamily regulates diverse biologic functions, including cell proliferation, differentiation, and survival, in addition to providing costimulatory signals for programmed cell death or apoptosis. In this study, cDNA fragments for two distinct TNFR homologues were obtained from a Japanese flounder, Paralichthys olivaceus, cDNA library. Full-length cDNAs of TNFR-1 and TNFR-2 homologues were obtained by using these cDNA fragments as probes. The cDNA for the Japanese flounder TNFR-1 homologue predicts a peptide of 395 amino acids that is 35% identical to the extracellular region of mouse TNFR-1, whereas the cDNA of the Japanese flounder TNFR-2 homologue predicts a peptide of 483 amino acids that is 40% identical to the extracellular region of human TNFR-2. The cytoplasmic domain contains a sequence that has the consensus motif of the death domain of the Japanese flounder TNFR-1 homologue. In a healthy fish, the mRNAs of both TNFR homologues were predominantly expressed in leukocytes, kidney, gill, and spleen. Expression of the Japanese flounder TNFR-1 homologue was induced in peripheral blood lymphocytes (PBLs) after stimulation with LPS (500μg/ml) for 1h, and TNFR-2 homologue was strongly induced in PBLs after stimulation with Con A (50μg/ml) and PMA (0.35μg/ml) for 3h. The different expression patterns of the two distinct TNFR homologues may be critical in determining whether binding with TNF-α or TNF-β have activating, proliferative, or apoptotic effects on target cells.

AB - Tumor necrosis factor receptor (TNFR) superfamily regulates diverse biologic functions, including cell proliferation, differentiation, and survival, in addition to providing costimulatory signals for programmed cell death or apoptosis. In this study, cDNA fragments for two distinct TNFR homologues were obtained from a Japanese flounder, Paralichthys olivaceus, cDNA library. Full-length cDNAs of TNFR-1 and TNFR-2 homologues were obtained by using these cDNA fragments as probes. The cDNA for the Japanese flounder TNFR-1 homologue predicts a peptide of 395 amino acids that is 35% identical to the extracellular region of mouse TNFR-1, whereas the cDNA of the Japanese flounder TNFR-2 homologue predicts a peptide of 483 amino acids that is 40% identical to the extracellular region of human TNFR-2. The cytoplasmic domain contains a sequence that has the consensus motif of the death domain of the Japanese flounder TNFR-1 homologue. In a healthy fish, the mRNAs of both TNFR homologues were predominantly expressed in leukocytes, kidney, gill, and spleen. Expression of the Japanese flounder TNFR-1 homologue was induced in peripheral blood lymphocytes (PBLs) after stimulation with LPS (500μg/ml) for 1h, and TNFR-2 homologue was strongly induced in PBLs after stimulation with Con A (50μg/ml) and PMA (0.35μg/ml) for 3h. The different expression patterns of the two distinct TNFR homologues may be critical in determining whether binding with TNF-α or TNF-β have activating, proliferative, or apoptotic effects on target cells.

KW - Apoptosis

KW - cDNA

KW - Con A/PMA

KW - Japanese flounder

KW - LPS

KW - Peripheral blood lymphocyte

KW - Tumor necrosis factor receptor

UR - http://www.scopus.com/inward/record.url?scp=0037400821&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037400821&partnerID=8YFLogxK

U2 - 10.1016/S0145-305X(02)00118-0

DO - 10.1016/S0145-305X(02)00118-0

M3 - Article

C2 - 12631519

AN - SCOPUS:0037400821

VL - 27

SP - 365

EP - 375

JO - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

SN - 0145-305X

IS - 5

ER -