Cloning and analysis of the promoter region of the human fibronectin gene

D. C. Dean, Christopher Bowlus, S. Bourgeois

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Human fibronectin (FN) genomic clones were isolated by screening a human genomic library with a 75-base oligonucleotide. The sequence of the oligonucleotide corresponds to a region near the 5' end of the human FN cDNA clone pFH6 that contains the amino-terminal coding sequences but does not extend to the 5' end of the mRNA [Kornblihtt, A.R., Umezawa, K., Vibe-Pedersen, K. & Baralle, F.E. (1985) EMBO J. 4, 1755-1759]. The 5' end of the FN gene is found on a 3.7-kilobase-pair EcoRI fragment that contains about 2.7 kilobase pairs of flanking sequence. The first exon is 414 base pairs long, with a 5' untranslated region of 267 base pairs. As deduced on the basis of the position of the initiation codon, FN is synthesized with a 31-residue amino acid extension on the amino terminus that is not present in the mature polypeptide. This amino-terminal extension appears to contain both a signal peptide and a propeptide. The first 200 base pairs of 5'-flanking sequence is very G+C rich. Upstream of this the sequence becomes relatively A+T rich. The sequence ATATAA is found at -25 and the sequence CAAT is present at -150. The sequence GGGGCGGGGC at -102 exhibits homology to the binding site for the transcription factor SP1, and the sequence TGACGTCA at -173 exhibits homology to 5'-flanking sequences important for induction by cAMP.

Original languageEnglish (US)
Pages (from-to)1876-1880
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number7
StatePublished - 1987
Externally publishedYes

Fingerprint

Fibronectins
Genetic Promoter Regions
Organism Cloning
Base Pairing
5' Flanking Region
Oligonucleotides
Genes
Clone Cells
Initiator Codon
Genomic Library
5' Untranslated Regions
Protein Sorting Signals
Exons
Transcription Factors
Complementary DNA
Binding Sites
Amino Acids
Messenger RNA
Peptides

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Cloning and analysis of the promoter region of the human fibronectin gene. / Dean, D. C.; Bowlus, Christopher; Bourgeois, S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, No. 7, 1987, p. 1876-1880.

Research output: Contribution to journalArticle

@article{3bdb8cc3029a493ab0b200ce25358308,
title = "Cloning and analysis of the promoter region of the human fibronectin gene",
abstract = "Human fibronectin (FN) genomic clones were isolated by screening a human genomic library with a 75-base oligonucleotide. The sequence of the oligonucleotide corresponds to a region near the 5' end of the human FN cDNA clone pFH6 that contains the amino-terminal coding sequences but does not extend to the 5' end of the mRNA [Kornblihtt, A.R., Umezawa, K., Vibe-Pedersen, K. & Baralle, F.E. (1985) EMBO J. 4, 1755-1759]. The 5' end of the FN gene is found on a 3.7-kilobase-pair EcoRI fragment that contains about 2.7 kilobase pairs of flanking sequence. The first exon is 414 base pairs long, with a 5' untranslated region of 267 base pairs. As deduced on the basis of the position of the initiation codon, FN is synthesized with a 31-residue amino acid extension on the amino terminus that is not present in the mature polypeptide. This amino-terminal extension appears to contain both a signal peptide and a propeptide. The first 200 base pairs of 5'-flanking sequence is very G+C rich. Upstream of this the sequence becomes relatively A+T rich. The sequence ATATAA is found at -25 and the sequence CAAT is present at -150. The sequence GGGGCGGGGC at -102 exhibits homology to the binding site for the transcription factor SP1, and the sequence TGACGTCA at -173 exhibits homology to 5'-flanking sequences important for induction by cAMP.",
author = "Dean, {D. C.} and Christopher Bowlus and S. Bourgeois",
year = "1987",
language = "English (US)",
volume = "84",
pages = "1876--1880",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "7",

}

TY - JOUR

T1 - Cloning and analysis of the promoter region of the human fibronectin gene

AU - Dean, D. C.

AU - Bowlus, Christopher

AU - Bourgeois, S.

PY - 1987

Y1 - 1987

N2 - Human fibronectin (FN) genomic clones were isolated by screening a human genomic library with a 75-base oligonucleotide. The sequence of the oligonucleotide corresponds to a region near the 5' end of the human FN cDNA clone pFH6 that contains the amino-terminal coding sequences but does not extend to the 5' end of the mRNA [Kornblihtt, A.R., Umezawa, K., Vibe-Pedersen, K. & Baralle, F.E. (1985) EMBO J. 4, 1755-1759]. The 5' end of the FN gene is found on a 3.7-kilobase-pair EcoRI fragment that contains about 2.7 kilobase pairs of flanking sequence. The first exon is 414 base pairs long, with a 5' untranslated region of 267 base pairs. As deduced on the basis of the position of the initiation codon, FN is synthesized with a 31-residue amino acid extension on the amino terminus that is not present in the mature polypeptide. This amino-terminal extension appears to contain both a signal peptide and a propeptide. The first 200 base pairs of 5'-flanking sequence is very G+C rich. Upstream of this the sequence becomes relatively A+T rich. The sequence ATATAA is found at -25 and the sequence CAAT is present at -150. The sequence GGGGCGGGGC at -102 exhibits homology to the binding site for the transcription factor SP1, and the sequence TGACGTCA at -173 exhibits homology to 5'-flanking sequences important for induction by cAMP.

AB - Human fibronectin (FN) genomic clones were isolated by screening a human genomic library with a 75-base oligonucleotide. The sequence of the oligonucleotide corresponds to a region near the 5' end of the human FN cDNA clone pFH6 that contains the amino-terminal coding sequences but does not extend to the 5' end of the mRNA [Kornblihtt, A.R., Umezawa, K., Vibe-Pedersen, K. & Baralle, F.E. (1985) EMBO J. 4, 1755-1759]. The 5' end of the FN gene is found on a 3.7-kilobase-pair EcoRI fragment that contains about 2.7 kilobase pairs of flanking sequence. The first exon is 414 base pairs long, with a 5' untranslated region of 267 base pairs. As deduced on the basis of the position of the initiation codon, FN is synthesized with a 31-residue amino acid extension on the amino terminus that is not present in the mature polypeptide. This amino-terminal extension appears to contain both a signal peptide and a propeptide. The first 200 base pairs of 5'-flanking sequence is very G+C rich. Upstream of this the sequence becomes relatively A+T rich. The sequence ATATAA is found at -25 and the sequence CAAT is present at -150. The sequence GGGGCGGGGC at -102 exhibits homology to the binding site for the transcription factor SP1, and the sequence TGACGTCA at -173 exhibits homology to 5'-flanking sequences important for induction by cAMP.

UR - http://www.scopus.com/inward/record.url?scp=0023317857&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023317857&partnerID=8YFLogxK

M3 - Article

C2 - 3031656

AN - SCOPUS:0023317857

VL - 84

SP - 1876

EP - 1880

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 7

ER -