Clones of Streptococcus zooepidemicus from outbreaks of hemorrhagic canine pneumonia and associated immune responses

Sridhar Velineni, John F. Timoney, Kim Russell, Heidi J. Hamlen, Patricia Pesavento, William D. Fortney, P. Cynda Crawford

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Acute hemorrhagic pneumonia caused by Streptococcus zooepidemicus has emerged as a major disease of shelter dogs and greyhounds. S. zooepidemicus strains differing in multilocus sequence typing (MLST), protective protein (SzP), and M-like protein (SzM) sequences were identified from 9 outbreaks in Texas, Kansas, Florida, Nevada, New Mexico, and Pennsylvania. Clonality based on 2 or more isolates was evident for 7 of these outbreaks. The Pennsylvania and Nevada outbreaks also involved cats. Goat antisera against acutely infected lung tissue as well as convalescent-phase sera reacted with a mucinase (Sz115), hyaluronidase (HylC), InlA domain-containing cell surface-anchored protein (INLA), membrane-anchored protein (MAP), SzP, SzM, and extracellular oligopeptide-binding protein (OppA). The amino acid sequences of SzP and SzM of the isolates varied greatly. The szp and szm alleles of the closely related Kansas clone (sequence type 129 [ST-129]) and United Kingdom isolate BHS5 (ST-123) were different, indicating that MLST was unreliable as a predictor of virulence phenotype. Combinations of conserved HylC and serine protease (ScpC) and variable SzM and SzP proteins of S. zooepidemicus strain NC78 were protectively immunogenic for mice challenged with a virulent canine strain. Thus, although canine pneumonia outbreaks are caused by different strains of S. zooepidemicus, protective immune responses were elicited in mice by combinations of conserved or variable S. zooepidemicus proteins from a single strain.

Original languageEnglish (US)
Pages (from-to)1246-1252
Number of pages7
JournalClinical and Vaccine Immunology
Volume21
Issue number9
DOIs
StatePublished - 2014

Fingerprint

Streptococcus equi
Disease Outbreaks
Canidae
Pneumonia
Clone Cells
Multilocus Sequence Typing
Protein S
hyaluronate lyase
Membrane Proteins
Dog Diseases
Oligopeptides
Proteins
Hyaluronoglucosaminidase
Serine Proteases
Goats
Virulence
Immune Sera
Amino Acid Sequence
Carrier Proteins
Cats

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

Clones of Streptococcus zooepidemicus from outbreaks of hemorrhagic canine pneumonia and associated immune responses. / Velineni, Sridhar; Timoney, John F.; Russell, Kim; Hamlen, Heidi J.; Pesavento, Patricia; Fortney, William D.; Crawford, P. Cynda.

In: Clinical and Vaccine Immunology, Vol. 21, No. 9, 2014, p. 1246-1252.

Research output: Contribution to journalArticle

Velineni, Sridhar ; Timoney, John F. ; Russell, Kim ; Hamlen, Heidi J. ; Pesavento, Patricia ; Fortney, William D. ; Crawford, P. Cynda. / Clones of Streptococcus zooepidemicus from outbreaks of hemorrhagic canine pneumonia and associated immune responses. In: Clinical and Vaccine Immunology. 2014 ; Vol. 21, No. 9. pp. 1246-1252.
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