Clinical evaluation of a rapid diagnostic test kit for detection of canine coronavirus

Seung Jae Yoon, Kyoung Won Seo, Kun Ho Song

Research output: Contribution to journalArticle

Abstract

Canine coronavirus is a single-stranded RNA virus that causes enteritis in dogs of any age. Coronaviral enteritis is seldom definitively diagnosed, since it is usually much less severe than many other types of enteritis and is self-limiting. Conventional diagnostics for the canine coronaviral enteritis such as polymerase chain reaction (PCR), virus isolation, and electron microscopic examination are inappropriate for small animal clinics due to the complicated experimental processes involved. Therefore, a commercially available lateral flow test kit based on chromatographic immunoassay techniques was tested to evaluate its performance as a first-line diagnostic test kit that could be used in clinics. The coronavirus antigen test kit detected canine coronavirus-infected dogs with 93.1% sensitivity and 97.5% specificity. The detection limit of the test kit was between 1.97 × 104/mL and 9.85 × 103/mL for samples with a 2- fold serial dilution from 1.25 × 106 TCID50 (TCID50, 50% tissue culture infectious dose). Additionally, the test kit had no cross-reactivity with canine parvovirus, distemper virus, or Escherichia coli. Overall, the commercially available test kit showed good diagnostic performance in a clinical setting, with results similar to those from PCR, confirming their potential for convenient and accurate use in small animal clinics.

Original languageEnglish (US)
Pages (from-to)27-31
Number of pages5
JournalKorean Journal of Veterinary Research
Volume58
Issue number1
DOIs
StatePublished - Jan 1 2018
Externally publishedYes

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Keywords

  • Antigen test kit
  • Coronavirus
  • Dogs
  • Polymerase chain reaction

ASJC Scopus subject areas

  • veterinary(all)

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