Clinical evaluation of a rapid diagnostic test kit for detection of canine coronavirus

Seung Jae Yoon, Kyoung Won Seo, Kun Ho Song

Research output: Contribution to journalArticle

Abstract

Canine coronavirus is a single-stranded RNA virus that causes enteritis in dogs of any age. Coronaviral enteritis is seldom definitively diagnosed, since it is usually much less severe than many other types of enteritis and is self-limiting. Conventional diagnostics for the canine coronaviral enteritis such as polymerase chain reaction (PCR), virus isolation, and electron microscopic examination are inappropriate for small animal clinics due to the complicated experimental processes involved. Therefore, a commercially available lateral flow test kit based on chromatographic immunoassay techniques was tested to evaluate its performance as a first-line diagnostic test kit that could be used in clinics. The coronavirus antigen test kit detected canine coronavirus-infected dogs with 93.1% sensitivity and 97.5% specificity. The detection limit of the test kit was between 1.97 × 104/mL and 9.85 × 103/mL for samples with a 2- fold serial dilution from 1.25 × 106 TCID50 (TCID50, 50% tissue culture infectious dose). Additionally, the test kit had no cross-reactivity with canine parvovirus, distemper virus, or Escherichia coli. Overall, the commercially available test kit showed good diagnostic performance in a clinical setting, with results similar to those from PCR, confirming their potential for convenient and accurate use in small animal clinics.

Original languageEnglish (US)
Pages (from-to)27-31
Number of pages5
JournalKorean Journal of Veterinary Research
Volume58
Issue number1
DOIs
StatePublished - Jan 1 2018
Externally publishedYes

Fingerprint

Canine Coronavirus
Diagnostic Reagent Kits
Canine coronavirus
analytical kits
Enteritis
diagnostic techniques
enteritis
Canine Parvovirus
Canine Distemper Virus
Dogs
Polymerase Chain Reaction
Coronavirus
dogs
RNA Viruses
polymerase chain reaction
Immunoassay
distemper
Limit of Detection
Canidae
immunoaffinity chromatography

Keywords

  • Antigen test kit
  • Coronavirus
  • Dogs
  • Polymerase chain reaction

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Clinical evaluation of a rapid diagnostic test kit for detection of canine coronavirus. / Yoon, Seung Jae; Seo, Kyoung Won; Song, Kun Ho.

In: Korean Journal of Veterinary Research, Vol. 58, No. 1, 01.01.2018, p. 27-31.

Research output: Contribution to journalArticle

@article{c3de8444792141f88d6df13df65a52e2,
title = "Clinical evaluation of a rapid diagnostic test kit for detection of canine coronavirus",
abstract = "Canine coronavirus is a single-stranded RNA virus that causes enteritis in dogs of any age. Coronaviral enteritis is seldom definitively diagnosed, since it is usually much less severe than many other types of enteritis and is self-limiting. Conventional diagnostics for the canine coronaviral enteritis such as polymerase chain reaction (PCR), virus isolation, and electron microscopic examination are inappropriate for small animal clinics due to the complicated experimental processes involved. Therefore, a commercially available lateral flow test kit based on chromatographic immunoassay techniques was tested to evaluate its performance as a first-line diagnostic test kit that could be used in clinics. The coronavirus antigen test kit detected canine coronavirus-infected dogs with 93.1{\%} sensitivity and 97.5{\%} specificity. The detection limit of the test kit was between 1.97 × 104/mL and 9.85 × 103/mL for samples with a 2- fold serial dilution from 1.25 × 106 TCID50 (TCID50, 50{\%} tissue culture infectious dose). Additionally, the test kit had no cross-reactivity with canine parvovirus, distemper virus, or Escherichia coli. Overall, the commercially available test kit showed good diagnostic performance in a clinical setting, with results similar to those from PCR, confirming their potential for convenient and accurate use in small animal clinics.",
keywords = "Antigen test kit, Coronavirus, Dogs, Polymerase chain reaction",
author = "Yoon, {Seung Jae} and Seo, {Kyoung Won} and Song, {Kun Ho}",
year = "2018",
month = "1",
day = "1",
doi = "10.14405/kjvr.2018.58.1.27",
language = "English (US)",
volume = "58",
pages = "27--31",
journal = "Korean Journal of Veterinary Research",
issn = "1225-0198",
publisher = "Korean Journal of Veterinary Research",
number = "1",

}

TY - JOUR

T1 - Clinical evaluation of a rapid diagnostic test kit for detection of canine coronavirus

AU - Yoon, Seung Jae

AU - Seo, Kyoung Won

AU - Song, Kun Ho

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Canine coronavirus is a single-stranded RNA virus that causes enteritis in dogs of any age. Coronaviral enteritis is seldom definitively diagnosed, since it is usually much less severe than many other types of enteritis and is self-limiting. Conventional diagnostics for the canine coronaviral enteritis such as polymerase chain reaction (PCR), virus isolation, and electron microscopic examination are inappropriate for small animal clinics due to the complicated experimental processes involved. Therefore, a commercially available lateral flow test kit based on chromatographic immunoassay techniques was tested to evaluate its performance as a first-line diagnostic test kit that could be used in clinics. The coronavirus antigen test kit detected canine coronavirus-infected dogs with 93.1% sensitivity and 97.5% specificity. The detection limit of the test kit was between 1.97 × 104/mL and 9.85 × 103/mL for samples with a 2- fold serial dilution from 1.25 × 106 TCID50 (TCID50, 50% tissue culture infectious dose). Additionally, the test kit had no cross-reactivity with canine parvovirus, distemper virus, or Escherichia coli. Overall, the commercially available test kit showed good diagnostic performance in a clinical setting, with results similar to those from PCR, confirming their potential for convenient and accurate use in small animal clinics.

AB - Canine coronavirus is a single-stranded RNA virus that causes enteritis in dogs of any age. Coronaviral enteritis is seldom definitively diagnosed, since it is usually much less severe than many other types of enteritis and is self-limiting. Conventional diagnostics for the canine coronaviral enteritis such as polymerase chain reaction (PCR), virus isolation, and electron microscopic examination are inappropriate for small animal clinics due to the complicated experimental processes involved. Therefore, a commercially available lateral flow test kit based on chromatographic immunoassay techniques was tested to evaluate its performance as a first-line diagnostic test kit that could be used in clinics. The coronavirus antigen test kit detected canine coronavirus-infected dogs with 93.1% sensitivity and 97.5% specificity. The detection limit of the test kit was between 1.97 × 104/mL and 9.85 × 103/mL for samples with a 2- fold serial dilution from 1.25 × 106 TCID50 (TCID50, 50% tissue culture infectious dose). Additionally, the test kit had no cross-reactivity with canine parvovirus, distemper virus, or Escherichia coli. Overall, the commercially available test kit showed good diagnostic performance in a clinical setting, with results similar to those from PCR, confirming their potential for convenient and accurate use in small animal clinics.

KW - Antigen test kit

KW - Coronavirus

KW - Dogs

KW - Polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=85045689639&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85045689639&partnerID=8YFLogxK

U2 - 10.14405/kjvr.2018.58.1.27

DO - 10.14405/kjvr.2018.58.1.27

M3 - Article

VL - 58

SP - 27

EP - 31

JO - Korean Journal of Veterinary Research

JF - Korean Journal of Veterinary Research

SN - 1225-0198

IS - 1

ER -