Abstract
Canine coronavirus is a single-stranded RNA virus that causes enteritis in dogs of any age. Coronaviral enteritis is seldom definitively diagnosed, since it is usually much less severe than many other types of enteritis and is self-limiting. Conventional diagnostics for the canine coronaviral enteritis such as polymerase chain reaction (PCR), virus isolation, and electron microscopic examination are inappropriate for small animal clinics due to the complicated experimental processes involved. Therefore, a commercially available lateral flow test kit based on chromatographic immunoassay techniques was tested to evaluate its performance as a first-line diagnostic test kit that could be used in clinics. The coronavirus antigen test kit detected canine coronavirus-infected dogs with 93.1% sensitivity and 97.5% specificity. The detection limit of the test kit was between 1.97 × 104/mL and 9.85 × 103/mL for samples with a 2- fold serial dilution from 1.25 × 106 TCID50 (TCID50, 50% tissue culture infectious dose). Additionally, the test kit had no cross-reactivity with canine parvovirus, distemper virus, or Escherichia coli. Overall, the commercially available test kit showed good diagnostic performance in a clinical setting, with results similar to those from PCR, confirming their potential for convenient and accurate use in small animal clinics.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 27-31 |
| Number of pages | 5 |
| Journal | Korean Journal of Veterinary Research |
| Volume | 58 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2018 |
| Externally published | Yes |
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Keywords
- Antigen test kit
- Coronavirus
- Dogs
- Polymerase chain reaction
ASJC Scopus subject areas
- veterinary(all)
Cite this
Clinical evaluation of a rapid diagnostic test kit for detection of canine coronavirus. / Yoon, Seung Jae; Seo, Kyoung Won; Song, Kun Ho.
In: Korean Journal of Veterinary Research, Vol. 58, No. 1, 01.01.2018, p. 27-31.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Clinical evaluation of a rapid diagnostic test kit for detection of canine coronavirus
AU - Yoon, Seung Jae
AU - Seo, Kyoung Won
AU - Song, Kun Ho
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Canine coronavirus is a single-stranded RNA virus that causes enteritis in dogs of any age. Coronaviral enteritis is seldom definitively diagnosed, since it is usually much less severe than many other types of enteritis and is self-limiting. Conventional diagnostics for the canine coronaviral enteritis such as polymerase chain reaction (PCR), virus isolation, and electron microscopic examination are inappropriate for small animal clinics due to the complicated experimental processes involved. Therefore, a commercially available lateral flow test kit based on chromatographic immunoassay techniques was tested to evaluate its performance as a first-line diagnostic test kit that could be used in clinics. The coronavirus antigen test kit detected canine coronavirus-infected dogs with 93.1% sensitivity and 97.5% specificity. The detection limit of the test kit was between 1.97 × 104/mL and 9.85 × 103/mL for samples with a 2- fold serial dilution from 1.25 × 106 TCID50 (TCID50, 50% tissue culture infectious dose). Additionally, the test kit had no cross-reactivity with canine parvovirus, distemper virus, or Escherichia coli. Overall, the commercially available test kit showed good diagnostic performance in a clinical setting, with results similar to those from PCR, confirming their potential for convenient and accurate use in small animal clinics.
AB - Canine coronavirus is a single-stranded RNA virus that causes enteritis in dogs of any age. Coronaviral enteritis is seldom definitively diagnosed, since it is usually much less severe than many other types of enteritis and is self-limiting. Conventional diagnostics for the canine coronaviral enteritis such as polymerase chain reaction (PCR), virus isolation, and electron microscopic examination are inappropriate for small animal clinics due to the complicated experimental processes involved. Therefore, a commercially available lateral flow test kit based on chromatographic immunoassay techniques was tested to evaluate its performance as a first-line diagnostic test kit that could be used in clinics. The coronavirus antigen test kit detected canine coronavirus-infected dogs with 93.1% sensitivity and 97.5% specificity. The detection limit of the test kit was between 1.97 × 104/mL and 9.85 × 103/mL for samples with a 2- fold serial dilution from 1.25 × 106 TCID50 (TCID50, 50% tissue culture infectious dose). Additionally, the test kit had no cross-reactivity with canine parvovirus, distemper virus, or Escherichia coli. Overall, the commercially available test kit showed good diagnostic performance in a clinical setting, with results similar to those from PCR, confirming their potential for convenient and accurate use in small animal clinics.
KW - Antigen test kit
KW - Coronavirus
KW - Dogs
KW - Polymerase chain reaction
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U2 - 10.14405/kjvr.2018.58.1.27
DO - 10.14405/kjvr.2018.58.1.27
M3 - Article
VL - 58
SP - 27
EP - 31
JO - Korean Journal of Veterinary Research
JF - Korean Journal of Veterinary Research
SN - 1225-0198
IS - 1
ER -