Click assembly of magnetic nanovectors for gene delivery

Souvik Biswas, Laura E. Gordon, Geoffrey J. Clark, Michael H. Nantz

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Functionalization of iron oxide nanoparticles with quaternary ammonium ion-based aminooxy and oxime ether substrates provides a flexible route for generating magnetic gene delivery vectors. Using the MCF-7 breast cancer cell line, our findings show that pDNA magnetoplexes derived from the lipid-coated nanoparticle formulation dMLP transfect in the presence of 10% serum with or without magnetic assistance at significantly higher levels than a commonly used cationic liposome formulation, based on luciferase assay. The present ion-pairing, click chemistry approach furnishes Fe3O4 nanoparticles with lipid layers. The resultant magnetic nanovectors serve as transfection enhancers for otherwise transfection-inactive materials.

Original languageEnglish (US)
Pages (from-to)2683-2688
Number of pages6
JournalBiomaterials
Volume32
Issue number10
DOIs
StatePublished - Apr 2011
Externally publishedYes

Keywords

  • Aminooxy
  • Magnetofection
  • Magnetoplex
  • Nanoparticle
  • Oximation

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

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  • Cite this

    Biswas, S., Gordon, L. E., Clark, G. J., & Nantz, M. H. (2011). Click assembly of magnetic nanovectors for gene delivery. Biomaterials, 32(10), 2683-2688. https://doi.org/10.1016/j.biomaterials.2010.12.047