Click assembly of magnetic nanovectors for gene delivery

Souvik Biswas, Laura E. Gordon, Geoffrey J. Clark, Michael H. Nantz

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Functionalization of iron oxide nanoparticles with quaternary ammonium ion-based aminooxy and oxime ether substrates provides a flexible route for generating magnetic gene delivery vectors. Using the MCF-7 breast cancer cell line, our findings show that pDNA magnetoplexes derived from the lipid-coated nanoparticle formulation dMLP transfect in the presence of 10% serum with or without magnetic assistance at significantly higher levels than a commonly used cationic liposome formulation, based on luciferase assay. The present ion-pairing, click chemistry approach furnishes Fe3O4 nanoparticles with lipid layers. The resultant magnetic nanovectors serve as transfection enhancers for otherwise transfection-inactive materials.

Original languageEnglish (US)
Pages (from-to)2683-2688
Number of pages6
JournalBiomaterials
Volume32
Issue number10
DOIs
StatePublished - Apr 2011
Externally publishedYes

Fingerprint

Nanoparticles
Genes
Lipids
Transfection
Click Chemistry
Ions
Oximes
Liposomes
Luciferases
Iron oxides
Ammonium Compounds
Ether
Ethers
Assays
Cells
Breast Neoplasms
Cell Line
Substrates
Serum
ferric oxide

Keywords

  • Aminooxy
  • Magnetofection
  • Magnetoplex
  • Nanoparticle
  • Oximation

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Biswas, S., Gordon, L. E., Clark, G. J., & Nantz, M. H. (2011). Click assembly of magnetic nanovectors for gene delivery. Biomaterials, 32(10), 2683-2688. https://doi.org/10.1016/j.biomaterials.2010.12.047

Click assembly of magnetic nanovectors for gene delivery. / Biswas, Souvik; Gordon, Laura E.; Clark, Geoffrey J.; Nantz, Michael H.

In: Biomaterials, Vol. 32, No. 10, 04.2011, p. 2683-2688.

Research output: Contribution to journalArticle

Biswas, S, Gordon, LE, Clark, GJ & Nantz, MH 2011, 'Click assembly of magnetic nanovectors for gene delivery', Biomaterials, vol. 32, no. 10, pp. 2683-2688. https://doi.org/10.1016/j.biomaterials.2010.12.047
Biswas, Souvik ; Gordon, Laura E. ; Clark, Geoffrey J. ; Nantz, Michael H. / Click assembly of magnetic nanovectors for gene delivery. In: Biomaterials. 2011 ; Vol. 32, No. 10. pp. 2683-2688.
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