Cleavage of cten by caspase-3 during apoptosis

Su Shun Lo, Su Huey Lo, Su Hao Lo

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Normal endothelial and epithelial cells undergo apoptosis when cell adhesion and spreading are disrupted, implying a critical role of focal adhesions in cell survival. Cten is a focal adhesion molecule of the tensin family. In contrast to other tensins, cten expression is limited to very few tissues, such as the prostate, and only in epithelial cells. Here, we have explored the potential roles of cten in apoptosis. We found cten was cleaved during apoptosis induced by staurosporine in normal prostate epithelial cells. By using recombinant caspases and site-directed mutagenesis, we have identified caspase-3 as the major protease to digest cten at the DSTD570↓S site. The biological relevance of cten-cleaved fragments was demonstrated by cells ectopically expressing these fragments. Cten fragment (residues 571-715) containing the phosphotyrosine-binding domain significantly reduced the growth rate. Detection of cleaved poly(ADP-ribose) polymerase and annexin binding in cells expressing cten (571-715) indicated that a fraction of cells underwent apoptosis. These results demonstrate that cten is a target of caspase-3 and the resultant fragments could further promote apoptosis.

Original languageEnglish (US)
Pages (from-to)4311-4314
Number of pages4
JournalOncogene
Volume24
Issue number26
DOIs
StatePublished - Jun 16 2005

    Fingerprint

Keywords

  • Apoptosis caspase
  • cten
  • Focal adhesion
  • Tensin

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this