Clear cell and endometrioid carcinomas: are their differences attributable to distinct cells of origin?

Dawn R. Cochrane, Basile Tessier-Cloutier, Katherine M. Lawrence, Tayyebeh Nazeran, Anthony Karnezis, Clara Salamanca, Angela S. Cheng, Jessica N. McAlpine, Lien N. Hoang, C. Blake Gilks, David G. Huntsman

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Endometrial epithelium is the presumed tissue of origin for both eutopic and endometriosis-derived clear cell and endometrioid carcinomas. We had previously hypothesized that the morphological, biological and clinical differences between these carcinomas are due to histotype-specific mutations. Although some mutations and genomic landscape features are more likely to be found in one of these histotypes, we were not able to identify a single class of mutations that was exclusively present in one histotype and not the other. This lack of genomic differences led us to an alternative hypothesis that these cancers could arise from distinct cells of origin within endometrial tissue, and that it is the cellular context that accounts for their differences. In a proteomic screen, we identified cystathionine γ-lyase (CTH) as a marker for clear cell carcinoma, as it is expressed at high levels in clear cell carcinomas of the ovary and endometrium. In the current study, we analysed normal Müllerian tissues, and found that CTH is expressed in ciliated cells of endometrium (both eutopic endometrium and endometriosis) and fallopian tubes. We then demonstrated that other ciliated cell markers are expressed in clear cell carcinomas, whereas endometrial secretory cell markers are expressed in endometrioid carcinomas. The same differential staining of secretory and ciliated cells was demonstrable in a three-dimensional organoid culture system, in which stem cells were stimulated to differentiate into an admixture of secretory and ciliated cells. These data suggest that endometrioid carcinomas are derived from cells of the secretory cell lineage, whereas clear cell carcinomas are derived from, or have similarities to, cells of the ciliated cell lineage.

Original languageEnglish (US)
Pages (from-to)26-36
Number of pages11
JournalJournal of Pathology
Volume243
Issue number1
DOIs
StatePublished - Sep 1 2017
Externally publishedYes

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Endometrioid Carcinoma
Cystathionine
Lyases
Cell Lineage
Endometriosis
Endometrial Neoplasms
Endometrium
Carcinoma
Mutation
Organoids
Fallopian Tubes
Proteomics

Keywords

  • ciliated cells
  • clear cell cancer
  • endometrioid cancer
  • endometriosis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Cochrane, D. R., Tessier-Cloutier, B., Lawrence, K. M., Nazeran, T., Karnezis, A., Salamanca, C., ... Huntsman, D. G. (2017). Clear cell and endometrioid carcinomas: are their differences attributable to distinct cells of origin? Journal of Pathology, 243(1), 26-36. https://doi.org/10.1002/path.4934

Clear cell and endometrioid carcinomas : are their differences attributable to distinct cells of origin? / Cochrane, Dawn R.; Tessier-Cloutier, Basile; Lawrence, Katherine M.; Nazeran, Tayyebeh; Karnezis, Anthony; Salamanca, Clara; Cheng, Angela S.; McAlpine, Jessica N.; Hoang, Lien N.; Gilks, C. Blake; Huntsman, David G.

In: Journal of Pathology, Vol. 243, No. 1, 01.09.2017, p. 26-36.

Research output: Contribution to journalArticle

Cochrane, DR, Tessier-Cloutier, B, Lawrence, KM, Nazeran, T, Karnezis, A, Salamanca, C, Cheng, AS, McAlpine, JN, Hoang, LN, Gilks, CB & Huntsman, DG 2017, 'Clear cell and endometrioid carcinomas: are their differences attributable to distinct cells of origin?', Journal of Pathology, vol. 243, no. 1, pp. 26-36. https://doi.org/10.1002/path.4934
Cochrane, Dawn R. ; Tessier-Cloutier, Basile ; Lawrence, Katherine M. ; Nazeran, Tayyebeh ; Karnezis, Anthony ; Salamanca, Clara ; Cheng, Angela S. ; McAlpine, Jessica N. ; Hoang, Lien N. ; Gilks, C. Blake ; Huntsman, David G. / Clear cell and endometrioid carcinomas : are their differences attributable to distinct cells of origin?. In: Journal of Pathology. 2017 ; Vol. 243, No. 1. pp. 26-36.
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AU - Cheng, Angela S.

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