Classification of aortic atherosclerotic lesions with time-resolved fluorescence spectroscopy

Jean Michel I Maarek, Laura Marcu, Warren S. Grundfest, Michael Fishbein

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

In this study, we examine the possibility of differentiating between classes of atherosclerotic lesions based on time-resolved fluorescence spectroscopy and we compare the performance of classification schemes that use either the time-resolved spectra or only the intensity spectra. Transient fluorescence emission induced by pulsed nitrogen laser excitation was measured on 87 excised samples of human aorta. The samples were classified histologically using the AHA classification (normal to type VI lesion). Predictor variables derived from the time-resolved spectra included the spectral intensities at 360-510 nm (10 nm interval) and parameters of a biexponential fit of the fluorescence impulse response function. Stepwise discriminant analysis using these predictor variables showed that a few predictor variables (time-dependent decay parameters at 390 nm, spectral intensities at 460 and 490 nm) sufficed to correctly classify 89% of the samples. Excluding the time-dependent decay and using only the spectral intensities, the percentage of correctly classified cases was significantly lower: 51%. These results establish that time-resolved fluorescence spectroscopy markedly improves on the performance of steady-state fluorescence spectroscopy for fine classification of atherosclerotic lesions.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages192-200
Number of pages9
Volume3600
StatePublished - 1999
Externally publishedYes
EventProceedings of the 1999 Biomedical Imaging: Reporters, Dyes, and Instrumentation - San Jose, CA, USA
Duration: Jan 26 1999Jan 28 1999

Other

OtherProceedings of the 1999 Biomedical Imaging: Reporters, Dyes, and Instrumentation
CitySan Jose, CA, USA
Period1/26/991/28/99

Fingerprint

Fluorescence spectroscopy
lesions
fluorescence
Fluorescence
spectroscopy
Laser excitation
Discriminant analysis
Impulse response
predictions
Nitrogen
nitrogen lasers
aorta
decay
impulses
pulsed lasers
intervals
excitation

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Maarek, J. M. I., Marcu, L., Grundfest, W. S., & Fishbein, M. (1999). Classification of aortic atherosclerotic lesions with time-resolved fluorescence spectroscopy. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 3600, pp. 192-200). Society of Photo-Optical Instrumentation Engineers.

Classification of aortic atherosclerotic lesions with time-resolved fluorescence spectroscopy. / Maarek, Jean Michel I; Marcu, Laura; Grundfest, Warren S.; Fishbein, Michael.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3600 Society of Photo-Optical Instrumentation Engineers, 1999. p. 192-200.

Research output: Chapter in Book/Report/Conference proceedingChapter

Maarek, JMI, Marcu, L, Grundfest, WS & Fishbein, M 1999, Classification of aortic atherosclerotic lesions with time-resolved fluorescence spectroscopy. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 3600, Society of Photo-Optical Instrumentation Engineers, pp. 192-200, Proceedings of the 1999 Biomedical Imaging: Reporters, Dyes, and Instrumentation, San Jose, CA, USA, 1/26/99.
Maarek JMI, Marcu L, Grundfest WS, Fishbein M. Classification of aortic atherosclerotic lesions with time-resolved fluorescence spectroscopy. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3600. Society of Photo-Optical Instrumentation Engineers. 1999. p. 192-200
Maarek, Jean Michel I ; Marcu, Laura ; Grundfest, Warren S. ; Fishbein, Michael. / Classification of aortic atherosclerotic lesions with time-resolved fluorescence spectroscopy. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3600 Society of Photo-Optical Instrumentation Engineers, 1999. pp. 192-200
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