Class II multiformity generated by variable MHC-DRB region configurations in the California sea lion (Zalophus californianus)

Lizabeth Bowen, Brian M. Aldridge, Frances Gulland, William Van Bonn, Robert DeLong, Sharon Melin, Linda J Lowenstine, Jeffrey L Stott, Michael L. Johnson

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

In light of the immunological importance of molecules encoded within the major histocompatibility complex (MHC), there are numerous studies examining the variability of these genes in wildlife populations. An underlying assumption in many of these studies is that MHC diversity invariably arises from a high level of allelic variation at a single gene locus, leading to widespread descriptions of thriving species with apparently limited MHC polymorphism. Indeed, in a previous study we failed to find sequence features compatible with traditionally diverse peptide-binding functions in MHC class II (DQA and DQB) genes in California sea lions and therefore expanded the search for polymorphism to the DRA and DRB genes. Our results show that, in contrast to Zaca-DQA, -DQB, and -DRA, Zaca-DRB has sequence features compatible with antigen binding and presentation. In fact Zaca-DRB constitutes a gene family, comprising at least seven loci, each of which exhibits limited variability, and which are present in variable configurations between individuals. This unusual mechanism for generating MHC DRB diversity is similar to that observed in the rhesus macaque, but has not been reported in any other species. The identification of a novel system of class II MHC variability in the California sea lion justifies new studies into the organizational basis of immunogenetic diversity in other marine species, and its role in infectious disease susceptibility.

Original languageEnglish (US)
Pages (from-to)12-27
Number of pages16
JournalImmunogenetics
Volume56
Issue number1
DOIs
StatePublished - Apr 2004

Fingerprint

Sea Lions
Dichlororibofuranosylbenzimidazole
Major Histocompatibility Complex
Genes
Immunogenetics
Disease Susceptibility
Antigen Presentation
Macaca mulatta
Communicable Diseases
Peptides
Population

Keywords

  • California sea lion
  • Comparative immunology
  • MHC
  • Veterinary immunology

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Bowen, L., Aldridge, B. M., Gulland, F., Van Bonn, W., DeLong, R., Melin, S., ... Johnson, M. L. (2004). Class II multiformity generated by variable MHC-DRB region configurations in the California sea lion (Zalophus californianus). Immunogenetics, 56(1), 12-27. https://doi.org/10.1007/s00251-004-0655-4

Class II multiformity generated by variable MHC-DRB region configurations in the California sea lion (Zalophus californianus). / Bowen, Lizabeth; Aldridge, Brian M.; Gulland, Frances; Van Bonn, William; DeLong, Robert; Melin, Sharon; Lowenstine, Linda J; Stott, Jeffrey L; Johnson, Michael L.

In: Immunogenetics, Vol. 56, No. 1, 04.2004, p. 12-27.

Research output: Contribution to journalArticle

Bowen, Lizabeth ; Aldridge, Brian M. ; Gulland, Frances ; Van Bonn, William ; DeLong, Robert ; Melin, Sharon ; Lowenstine, Linda J ; Stott, Jeffrey L ; Johnson, Michael L. / Class II multiformity generated by variable MHC-DRB region configurations in the California sea lion (Zalophus californianus). In: Immunogenetics. 2004 ; Vol. 56, No. 1. pp. 12-27.
@article{b31728a8d6174c1789c32cb83b76e25e,
title = "Class II multiformity generated by variable MHC-DRB region configurations in the California sea lion (Zalophus californianus)",
abstract = "In light of the immunological importance of molecules encoded within the major histocompatibility complex (MHC), there are numerous studies examining the variability of these genes in wildlife populations. An underlying assumption in many of these studies is that MHC diversity invariably arises from a high level of allelic variation at a single gene locus, leading to widespread descriptions of thriving species with apparently limited MHC polymorphism. Indeed, in a previous study we failed to find sequence features compatible with traditionally diverse peptide-binding functions in MHC class II (DQA and DQB) genes in California sea lions and therefore expanded the search for polymorphism to the DRA and DRB genes. Our results show that, in contrast to Zaca-DQA, -DQB, and -DRA, Zaca-DRB has sequence features compatible with antigen binding and presentation. In fact Zaca-DRB constitutes a gene family, comprising at least seven loci, each of which exhibits limited variability, and which are present in variable configurations between individuals. This unusual mechanism for generating MHC DRB diversity is similar to that observed in the rhesus macaque, but has not been reported in any other species. The identification of a novel system of class II MHC variability in the California sea lion justifies new studies into the organizational basis of immunogenetic diversity in other marine species, and its role in infectious disease susceptibility.",
keywords = "California sea lion, Comparative immunology, MHC, Veterinary immunology",
author = "Lizabeth Bowen and Aldridge, {Brian M.} and Frances Gulland and {Van Bonn}, William and Robert DeLong and Sharon Melin and Lowenstine, {Linda J} and Stott, {Jeffrey L} and Johnson, {Michael L.}",
year = "2004",
month = "4",
doi = "10.1007/s00251-004-0655-4",
language = "English (US)",
volume = "56",
pages = "12--27",
journal = "Immunogenetics",
issn = "0093-7711",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Class II multiformity generated by variable MHC-DRB region configurations in the California sea lion (Zalophus californianus)

AU - Bowen, Lizabeth

AU - Aldridge, Brian M.

AU - Gulland, Frances

AU - Van Bonn, William

AU - DeLong, Robert

AU - Melin, Sharon

AU - Lowenstine, Linda J

AU - Stott, Jeffrey L

AU - Johnson, Michael L.

PY - 2004/4

Y1 - 2004/4

N2 - In light of the immunological importance of molecules encoded within the major histocompatibility complex (MHC), there are numerous studies examining the variability of these genes in wildlife populations. An underlying assumption in many of these studies is that MHC diversity invariably arises from a high level of allelic variation at a single gene locus, leading to widespread descriptions of thriving species with apparently limited MHC polymorphism. Indeed, in a previous study we failed to find sequence features compatible with traditionally diverse peptide-binding functions in MHC class II (DQA and DQB) genes in California sea lions and therefore expanded the search for polymorphism to the DRA and DRB genes. Our results show that, in contrast to Zaca-DQA, -DQB, and -DRA, Zaca-DRB has sequence features compatible with antigen binding and presentation. In fact Zaca-DRB constitutes a gene family, comprising at least seven loci, each of which exhibits limited variability, and which are present in variable configurations between individuals. This unusual mechanism for generating MHC DRB diversity is similar to that observed in the rhesus macaque, but has not been reported in any other species. The identification of a novel system of class II MHC variability in the California sea lion justifies new studies into the organizational basis of immunogenetic diversity in other marine species, and its role in infectious disease susceptibility.

AB - In light of the immunological importance of molecules encoded within the major histocompatibility complex (MHC), there are numerous studies examining the variability of these genes in wildlife populations. An underlying assumption in many of these studies is that MHC diversity invariably arises from a high level of allelic variation at a single gene locus, leading to widespread descriptions of thriving species with apparently limited MHC polymorphism. Indeed, in a previous study we failed to find sequence features compatible with traditionally diverse peptide-binding functions in MHC class II (DQA and DQB) genes in California sea lions and therefore expanded the search for polymorphism to the DRA and DRB genes. Our results show that, in contrast to Zaca-DQA, -DQB, and -DRA, Zaca-DRB has sequence features compatible with antigen binding and presentation. In fact Zaca-DRB constitutes a gene family, comprising at least seven loci, each of which exhibits limited variability, and which are present in variable configurations between individuals. This unusual mechanism for generating MHC DRB diversity is similar to that observed in the rhesus macaque, but has not been reported in any other species. The identification of a novel system of class II MHC variability in the California sea lion justifies new studies into the organizational basis of immunogenetic diversity in other marine species, and its role in infectious disease susceptibility.

KW - California sea lion

KW - Comparative immunology

KW - MHC

KW - Veterinary immunology

UR - http://www.scopus.com/inward/record.url?scp=2442604436&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442604436&partnerID=8YFLogxK

U2 - 10.1007/s00251-004-0655-4

DO - 10.1007/s00251-004-0655-4

M3 - Article

C2 - 14997355

AN - SCOPUS:2442604436

VL - 56

SP - 12

EP - 27

JO - Immunogenetics

JF - Immunogenetics

SN - 0093-7711

IS - 1

ER -