CLASPs function redundantly to regulate astral microtubules in the C. elegans embryo.

Eugenel B. Espiritu, Lori E. Krueger, Anna Ye, Lesilee S. Rose

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Microtubule dynamics are thought to play an important role in regulating microtubule interactions with cortical force generating motor proteins that position the spindle during asymmetric cell division. CLASPs are microtubule-associated proteins that have a conserved role in regulating microtubule dynamics in diverse cell types. Caenorhabditis elegans has three CLASP homologs in its genome. CLS-2 is known to localize to kinetochores and is needed for chromosome segregation at meiosis and mitosis; however CLS-1 and CLS-3 have not been reported to have any role in embryonic development. Here, we show that depletion of CLS-2 in combination with either CLS-1 or CLS-3 results in defects in nuclear rotation, maintenance of spindle length, and spindle displacement in the one-cell embryo. Polarity is normal in these embryos, but reduced numbers of astral microtubules reach all regions of the cortex at the time of spindle positioning. Analysis of the microtubule plus-end tracker EB1 also revealed a reduced number of growing microtubules reaching the cortex in CLASP depleted embryos, but the polymerization rate of astral microtubules was not slower than in wild type. These results indicate that C. elegans CLASPs act partially redundantly to regulate astral microtubules and position the spindle during asymmetric cell division. Further, we show that these spindle pole-positioning roles are independent of the CLS-2 binding proteins HCP-1 and HCP-2.

Original languageEnglish (US)
Pages (from-to)242-254
Number of pages13
JournalDevelopmental Biology
Volume368
Issue number2
DOIs
StatePublished - Aug 15 2012
Externally publishedYes

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Microtubules
Embryonic Structures
Asymmetric Cell Division
Caenorhabditis elegans
Spindle Poles
Kinetochores
Chromosome Segregation
Microtubule-Associated Proteins
Meiosis
Mitosis
Polymerization
Embryonic Development
Carrier Proteins
Maintenance
Genome
Proteins

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

CLASPs function redundantly to regulate astral microtubules in the C. elegans embryo. / Espiritu, Eugenel B.; Krueger, Lori E.; Ye, Anna; Rose, Lesilee S.

In: Developmental Biology, Vol. 368, No. 2, 15.08.2012, p. 242-254.

Research output: Contribution to journalArticle

Espiritu, Eugenel B. ; Krueger, Lori E. ; Ye, Anna ; Rose, Lesilee S. / CLASPs function redundantly to regulate astral microtubules in the C. elegans embryo. In: Developmental Biology. 2012 ; Vol. 368, No. 2. pp. 242-254.
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