Cigarette smoke exposure inhibits early phase of antibody production through inhibition of immune functions in alveolar macrophage

Kengo Kobayashi, Yuriko Hirono, Honami Nakta, Kent E. Pinkerton, Minoru Takeuchi

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Cigarette smoke (CS) is inhaled into the lung. Alveolar macrophage (AM) is known to play an important role in the lung immune system. However, the relationship between AM functions and antibody production by CS is not fully investigated. Objective: Therefore, we investigated the effects of AM from CS exposed mice on antibody pro-duction. Mice were exposed to 20 cigarettes/day for 10 days. AM were obtained by broncho-alveo-lar lavage. Antibody production was analyzed by plaque-forming cell assay using seep red blood cell (SRBC) as antigen. Methods: B cell proliferation was analyzed by3H-thymidine incorporation. Phagocytic activity using fluorescein isothiocyanate-labeled SRBC and expressions of surface antigens on AM were analyzed by flow cytometry. Cytokines and NF-κB mRNA expressions of AM were analyzed by RT-PCR. Results and Discussion: Antibody production was decreased at the induction phase, but not at the expression phase by AM from smoked mice (SM) compared with non-smoked mice (NSM). B cell proliferation was decreased by cigarette extracts dose-dependently. Phagocytic activity of AM was decreased in SM compared with NSM. Expression of surface antigens on AM was decreased in SM compared with NSM. Cytokines or NF-κB mRNA expressions of AM were decreased in SM compared with NSM. Conclusion: These results suggest that the inhibition of antibody production by cigarette smoking is caused by the inhibition of phagocytosis and expressions of surface antigens associated with antigen presentation. Such inhibition of AM functions may increase the risk of bacterial and virus infec-tions.

Original languageEnglish (US)
Pages (from-to)193-200
Number of pages8
JournalCurrent Respiratory Medicine Reviews
Volume16
Issue number3
DOIs
StatePublished - 2020

Keywords

  • Alveolar macrophage
  • Antibody production
  • Antigen presentation
  • Cigarette smoke
  • Cytokines
  • Phagocytosis

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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