Cigarette smoke exposure and hypercholesterolemia increase mitochondrial damage in cardiovascular tissues

Cynthia A. Knight-Lozano, Christal G. Young, David L. Burow, Zhao Yong Hu, Dale Uyeminami, Kent E Pinkerton, Harry Ischiropoulos, Scott W. Ballinger

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

Background - A shared feature among cardiovascular disease risk factors is increased oxidative stress. Because mitochondria are susceptible to damage mediated by oxidative stress, we hypothesized that risk factors (secondhand smoke and hypercholesterolemia) are associated with increased mitochondrial damage in cardiovascular tissues. Methods and Results - Atherosclerotic lesion formation, mitochondrial DNA damage, protein nitration, and specific activities of mitochondrial proteins in cardiovascular tissues from age-matched C57 and apoE-/- mice exposed to filtered air or secondhand smoke were quantified. Both secondhand smoke and hypercholesterolemia were associated with significantly increased mitochondrial DNA damage and protein nitration. Tobacco smoke exposure also resulted in significantly decreased specific activities of mitochondrial enzymes. The combination of secondhand smoke and hypercholesterolemia resulted in increased atherosclerotic lesion formation and even greater levels of mitochondrial damage. Conclusions - These data are consistent with the hypothesis that cardiovascular disease risk factors cause mitochondrial damage and dysfunction.

Original languageEnglish (US)
Pages (from-to)849-854
Number of pages6
JournalCirculation
Volume105
Issue number7
DOIs
StatePublished - Feb 19 2002

Fingerprint

Tobacco Smoke Pollution
Hypercholesterolemia
Smoke
Tobacco Products
Mitochondrial Proteins
Mitochondrial DNA
DNA Damage
Oxidative Stress
Cardiovascular Diseases
Apolipoproteins E
Tobacco
Mitochondria
Air
Enzymes
Proteins

Keywords

  • Atherosclerosis
  • Hypercholesterolemia
  • Mitochondria
  • Smoking

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Knight-Lozano, C. A., Young, C. G., Burow, D. L., Hu, Z. Y., Uyeminami, D., Pinkerton, K. E., ... Ballinger, S. W. (2002). Cigarette smoke exposure and hypercholesterolemia increase mitochondrial damage in cardiovascular tissues. Circulation, 105(7), 849-854. https://doi.org/10.1161/hc0702.103977

Cigarette smoke exposure and hypercholesterolemia increase mitochondrial damage in cardiovascular tissues. / Knight-Lozano, Cynthia A.; Young, Christal G.; Burow, David L.; Hu, Zhao Yong; Uyeminami, Dale; Pinkerton, Kent E; Ischiropoulos, Harry; Ballinger, Scott W.

In: Circulation, Vol. 105, No. 7, 19.02.2002, p. 849-854.

Research output: Contribution to journalArticle

Knight-Lozano, CA, Young, CG, Burow, DL, Hu, ZY, Uyeminami, D, Pinkerton, KE, Ischiropoulos, H & Ballinger, SW 2002, 'Cigarette smoke exposure and hypercholesterolemia increase mitochondrial damage in cardiovascular tissues', Circulation, vol. 105, no. 7, pp. 849-854. https://doi.org/10.1161/hc0702.103977
Knight-Lozano, Cynthia A. ; Young, Christal G. ; Burow, David L. ; Hu, Zhao Yong ; Uyeminami, Dale ; Pinkerton, Kent E ; Ischiropoulos, Harry ; Ballinger, Scott W. / Cigarette smoke exposure and hypercholesterolemia increase mitochondrial damage in cardiovascular tissues. In: Circulation. 2002 ; Vol. 105, No. 7. pp. 849-854.
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AU - Knight-Lozano, Cynthia A.

AU - Young, Christal G.

AU - Burow, David L.

AU - Hu, Zhao Yong

AU - Uyeminami, Dale

AU - Pinkerton, Kent E

AU - Ischiropoulos, Harry

AU - Ballinger, Scott W.

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N2 - Background - A shared feature among cardiovascular disease risk factors is increased oxidative stress. Because mitochondria are susceptible to damage mediated by oxidative stress, we hypothesized that risk factors (secondhand smoke and hypercholesterolemia) are associated with increased mitochondrial damage in cardiovascular tissues. Methods and Results - Atherosclerotic lesion formation, mitochondrial DNA damage, protein nitration, and specific activities of mitochondrial proteins in cardiovascular tissues from age-matched C57 and apoE-/- mice exposed to filtered air or secondhand smoke were quantified. Both secondhand smoke and hypercholesterolemia were associated with significantly increased mitochondrial DNA damage and protein nitration. Tobacco smoke exposure also resulted in significantly decreased specific activities of mitochondrial enzymes. The combination of secondhand smoke and hypercholesterolemia resulted in increased atherosclerotic lesion formation and even greater levels of mitochondrial damage. Conclusions - These data are consistent with the hypothesis that cardiovascular disease risk factors cause mitochondrial damage and dysfunction.

AB - Background - A shared feature among cardiovascular disease risk factors is increased oxidative stress. Because mitochondria are susceptible to damage mediated by oxidative stress, we hypothesized that risk factors (secondhand smoke and hypercholesterolemia) are associated with increased mitochondrial damage in cardiovascular tissues. Methods and Results - Atherosclerotic lesion formation, mitochondrial DNA damage, protein nitration, and specific activities of mitochondrial proteins in cardiovascular tissues from age-matched C57 and apoE-/- mice exposed to filtered air or secondhand smoke were quantified. Both secondhand smoke and hypercholesterolemia were associated with significantly increased mitochondrial DNA damage and protein nitration. Tobacco smoke exposure also resulted in significantly decreased specific activities of mitochondrial enzymes. The combination of secondhand smoke and hypercholesterolemia resulted in increased atherosclerotic lesion formation and even greater levels of mitochondrial damage. Conclusions - These data are consistent with the hypothesis that cardiovascular disease risk factors cause mitochondrial damage and dysfunction.

KW - Atherosclerosis

KW - Hypercholesterolemia

KW - Mitochondria

KW - Smoking

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