Chronology of labyrinthitis ossificans induced by Streptococcus pneumoniae meningitis

Vishad Nabili, Hilary A Brodie, Nikita I. Neverov, Steven P. Tinling

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Objective: Labyrinthitis ossificans consists of novel osteogenesis that fills the normally patent cochlear and vestibular lumen as an end-stage sequelae to various pathologies. This study was designed to establish the sequence of events and chronology of the osteoneogenesis and calcification. Study Design: A prospective randomized double-blind study. Methods: By using serial application of different colored fluorochromes, which deposit in newly forming bone, the timing of bone deposition and bone remodeling can be established. Labyrinthitis ossificans was induced in six groups (n = 5) of gerbils by an intrathecal injection of live Streptococcus pneumoniae. Group 1 received no fluorochrome labels, group 2 received one label, group 3 received three labels, and groups 4, 5, and 6 received four labels. The temporal bones were harvested after 2 weeks (group 1), 1 month (group 2), 3 months (group 3), 4 months (group 4), 6 months (group 5), and 12 months (group 6). Results: Sixteen of the 25 animals that received labels developed ossification, demonstrated with fluorescent microscopy. In the animals that developed labyrinthitis ossificans, newly formed disorganized bone began calcifying as early as 3 weeks (label 1) after S pneumoniae injection. Osteoneogenesis continued as evidenced by the presence of the other labels when first applied at 6 weeks (label 2), and 10 weeks (label 3). Ossification, calcification, and remodeling proceeded through a 12-month course, wherein a reduction of labels was present at 6 months and total disappearance by 12 months. Conclusions: The use of fluorescent stains in this animal model provides a means to establish a timeline of the ossification seen in labyrinthitis ossificans.

Original languageEnglish (US)
Pages (from-to)931-935
Number of pages5
JournalLaryngoscope
Volume109
Issue number6
DOIs
StatePublished - Jun 1999

Fingerprint

Labyrinthitis
Pneumococcal Meningitis
Chronology
Osteogenesis
Fluorescent Dyes
Bone and Bones
Spinal Injections
Gerbillinae
Bone Remodeling
Temporal Bone
Cochlea
Streptococcus pneumoniae
Double-Blind Method
Microscopy
Pneumonia
Coloring Agents
Animal Models
Pathology
Injections

Keywords

  • Cochlear implant
  • Deafness
  • Labyrinthitis
  • Meningitis
  • Ossified cochlea

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Chronology of labyrinthitis ossificans induced by Streptococcus pneumoniae meningitis. / Nabili, Vishad; Brodie, Hilary A; Neverov, Nikita I.; Tinling, Steven P.

In: Laryngoscope, Vol. 109, No. 6, 06.1999, p. 931-935.

Research output: Contribution to journalArticle

Nabili, Vishad ; Brodie, Hilary A ; Neverov, Nikita I. ; Tinling, Steven P. / Chronology of labyrinthitis ossificans induced by Streptococcus pneumoniae meningitis. In: Laryngoscope. 1999 ; Vol. 109, No. 6. pp. 931-935.
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